Results 61 to 70 of about 2,369,817 (302)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Assessment of condition of underground collector lines situated inside the technological complexes of underground storage facilities [PDF]

open access: yesActa Montanistica Slovaca, 2006
The evaluation of status of underground gas pipeline systems operating for several decades becomes a decisive factor of the decision making for their further safe and reliable operation.
Anton Misany   +2 more
doaj  

School storm shelter study report : to the 133rd Ohio General Assembly

open access: yes, 2019
"December 2019."; Includes bibliographical referencesIntroduction -- Consultation with stakeholders -- Building code requirements for school storm shelters -- Storm shelter cost impact on OFCC projects -- 2018 moratorium -- 2019 moratorium and study ...
Ohio Facilities Construction Commission
core  

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Propane Refueling at Facilities Services [PDF]

open access: yes, 2005
4 p.
University of Oregon. Campus Operations. Facilities Services
core   +1 more source

Texas Facilities Master Plan Report

open access: yes, 2012
"The purpose of this document is to apprise State leadership of agencies' current and projected facility requirements; recommend salient, cost effective initiatives to meet those needs; and establish a long-term asset management and development strategy ...
Texas Facilities Commission
core  

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Review of the progress of Sinopec gas storage construction and the next development direction

open access: yesDuankuai youqitian
Gas storage is a key infrastructure to ensure national energy security and optimize natural gas production, supply, reserve and sales system, and its construction achievements have attracted much attention.
SUN Wei
doaj   +1 more source

Numerical simulation of underground gas storage in an inclined aquifer

open access: yesНаука. Инновации. Технологии, 2022
Introduction. Underground gas storage plays an important role in changing gas consumption. The main factors in the creation of UGS facilities are the presence of large gas consumers and the availability of the necessary reservoirs.
S. Mulyavin   +3 more
doaj  

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