Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Rejection or support? research on labor participation strategy of older adults based on three-party evolutionary game. [PDF]
PLoS OneShen Y, Zhong Y, Sun F.
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Executive self-compassion, strategic inertia, and corporate performance. [PDF]
Front PsycholYu T, Liu N, Wang Y.
europepmc +1 more source
Why human connection is the true metric of research success
FEBS Open Bio, EarlyView.Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan +3 more
wiley +1 more source
Sequencing AI Automation and Data Interoperability in Oncology Using a Scenario-Planning Framework Coupled With Discrete-Event Simulation: Proof-of-Concept Study. [PDF]
J Med Internet ResMay P +3 more
europepmc +1 more source
Corporate entrepreneurship: Linking strategic roles to multiple dimensions of performance [PDF]
Using data from a large European financial services firm which engaged in an entrepreneurial initiative to enhance its competitiveness, this paper explores the strategic role of middle managers in the context of corporate entrepreneurship and its link to
Rata, Cristina, Mair, Johanna
core
FEBS Open Bio, EarlyView.A novel signature integrating genome‐wide analysis with clinical factors predicts recurrence in stage II colorectal cancer and enables a new risk stratification to guide postoperative adjuvant chemotherapy. Clinical risk stratification for postoperative recurrence in patients with pathological stage II (pStage II) colorectal cancer (CRC) is essential ...
Mayuko Otomo +7 more
wiley +1 more source
Analysis of Boron-Based and Rare-Earth-Based Additive Strategies in Advanced Oxide Materials in Terms of Structural-Morphological Performance and Critical Raw Material Policies. [PDF]
Nanomaterials (Basel)Gür B, Yaman H, Macit CK.
europepmc +1 more source