Results 141 to 150 of about 9,607 (263)

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

open access: yesCytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

open access: yesCytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen   +2 more
wiley   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

open access: yesCytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham   +3 more
wiley   +1 more source

MultNAT HPV16/18 Assay: A Simple, Rapid, and Low‐Cost Polymerase Chain Reaction Method for the Detection of HPV16 and HPV18 Suitable for Point‐of‐Care Testing

open access: yesCancer Nexus, EarlyView.
ABSTRACT Aim Persistent high‐risk HPV infection can lead to the development of precancerous lesions, potentially progressing to invasive cancer. HPV status serves not only as a prognostic marker for squamous cell carcinoma (HNSCC) like oropharyngeal squamous cell carcinomas (OPSCC) but also significantly influences clinical management and treatment ...
Min Wang   +9 more
wiley   +1 more source

Gut microbiota shifts and short‐chain fatty acids alterations in pediatric epilepsy patients on a Mediterranean ketogenic diet

open access: yesEpileptic Disorders, EarlyView.
Abstract Objective The olive oil–based Mediterranean ketogenic diet (MedKD) may support patients with drug‐resistant epilepsy (DRE) or neurometabolic disorders by integrating ketogenic therapy with the cardiometabolic and neuroprotective advantages of the Mediterranean diet.
Sofia Zouganeli   +8 more
wiley   +1 more source

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