Results 71 to 80 of about 326,672 (264)

Introduction to the special issue of the Consortium of Organizations for Strong Motion Observation Systems (COSMOS) international guidelines for applying noninvasive geophysical techniques to characterize seismic site conditions. [PDF]

J Seismol, 2022
Yong A, Askan A, Cassidy J, D'Amico S, Parolai S, Pilz M, Stephenson W.   +6 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Frecuency-wavenumber analysis of strong ground motion in Mexico City

Geofísica Internacional, 2017
Se presentan los resultados del análisis de las densidades espectrales de potencia en el dominio frecuencia-número de onda (f-k) de los movimientos sísmicos registrados por la Red Acelerométrica de la Ciudad de México (MCAN, por sus siglas en inglés). En
Marcela Baena-Rivera, L. Eduardo Pérez-Rocha, Francisco J. Sánchez-Sesma   +2 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Estimation of 1-D velocity models beneath strong-motion observation sites in the Kathmandu Valley using strong-motion records from moderate-sized earthquakes

Earth, Planets and Space, 2017
The Himalayan collision zone experiences many seismic activities with large earthquakes occurring at certain time intervals. The damming of the proto-Bagmati River as a result of rapid mountain-building processes created a lake in the Kathmandu Valley ...
Subeg M. Bijukchhen, Nobuo Takai, Michiko Shigefuji, Masayoshi Ichiyanagi, Tsutomu Sasatani, Yokito Sugimura   +5 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

The INGV real time strong motion data sharing during the 2016 Amatrice (central Italy) seismic sequence

Annals of Geophysics, 2016
ISMD is the real time INGV Strong Motion database. During the recent August-September 2016 Amatrice, Mw 6.0, seismic sequence, ISMD represented the main tool for the INGV real time strong motion data sharing.  Starting from August 24th,  the main task of
Marco Massa, Ezio D'Alema, Chiara Mascandola, Sara Lovati, Davide Scafidi, Gianlorenzo Franceschina, Antonio Gomez, Simona Carannante, Davide Piccaredda, Santi Mirenna, Paolo Augliera   +10 more
doaj   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source