Results 31 to 40 of about 50,984 (192)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Control of the persistent currents in two interacting quantum rings through the Coulomb interaction and inter-ring tunneling

, 2008
The persistent current in two vertically coupled quantum rings containing few electrons is studied. We find that the Coulomb interaction between the rings in the absence of tunneling affects the persistent current in each ring and the ground state ...
B. Partoens, F. M. Peeters, G.-Q. Hai, J. Hamilton, L. K. Castelano   +4 more
core   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Study of intermediate velocity products in the Ar+Ni collisions between 52 and 95 A.MeV

, 1999
Intermediate velocity products in Ar+Ni collisions from 52 to 95 A.MeV are studied in an experiment performed at the GANIL facility with the 4$\pi$ multidetector INDRA.
A. Chbihi, A. Demeyer, A. Le Fèvre, A. Rahmani, A. Wieloch, A.D. N'Guyen, A.M. Maskay, Albergo, Angélique, B. Hurst, B. Tamain, Beaulieu, Bertsch, Borderie, Buta, C. Volant, Casini, Ch.O. Bacri, Chen, Chen, Colonna, Colonna, Cussol, D. Cussol, D. Doré, D. Durand, D. Gourio, D. Guinet, Danielewicz, Dayras, De La Mota, Dempsey, Doss, Durand, E. Galichet, E. Genouin-Duhamel, E. Gerlic, E. Plagnol, E. Rosato, E. Vient, Eudes, F. Bocage, F. Saint-Laurent, Fuchs, G. Auger, G. Politi, G. Tabacaru, Gosset, Gulminelli, Gustafsson, Gutbrod, Hauger, He, J. Colin, J. Péter, J.C. Steckmeyer, J.D. Frankland, J.F. Lecolley, J.L. Charvet, J.L. Laville, J.P. Wieleczko, Jacak, Krofcheck, Kwiatkowski, L. Nalpas, L. Tassan-Got, Lanzano, Larochelle, Lecolley, Lefort, Li, Lukasik, M. Assenard, M. Germain, M. Louvel, M. Parlog, M. Stern, Ma, Marie, Milkau, Montoya, Moretto, Moretto, N. Le Neindre, Natowitz, O. Lopez, O. Tirel, Ogilvie, Ono, P. Eudes, P. Lautesse, Pak, Peilert, Ph. Buchet, Pochodzalla, Popescu, Pouthas, Pouthas, Péter, Péter, Péter, R. Bougault, R. Brou, R. Dayras, R. Legrain, Rivet, S Goldhaber, S. Salou, Sauvestre, Scheid, Shen, Siwek, Sobotka, Steckmeyer, Stefanini, Stuttgé, Stöcker, Sullivan, T. Lefort, T. Reposeur, Tsang, Tõke, Westfall, Westfall, Wile, Xi, Xu, Y.G. Ma, Zhang   +128 more
core   +3 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Electron-acoustic-phonon scattering and electron relaxation in two-coupled quantum rings

, 2007
Electron relaxation, induced by acoustic phonons, is studied for coupled quantum rings in the presence of external fields, both electric and magnetic. We address the problem of a single electron in vertically coupled GaAs quantum rings.
Hai, G. Q., Piacente, G.
core   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Chiral four-body interactions in nuclear matter

, 2012
An exploratory study of chiral four-nucleon interactions in nuclear and neutron matter is performed. The leading-order terms arising from pion-exchange in combination with the chiral $4\pi$-vertex and the chiral NN$3\pi$-vertex are found to be very small.
Kaiser, N.
core   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

On Polynomial Rings Over Nil Rings in Several Variables and the Central Closure of Prime Nil Rings

, 2016
We prove that the ring of polynomials in several commuting indeterminates over a nil ring cannot be homomorphically mapped onto a ring with identity, i.e. it is Brown-McCoy radical. It answers a question posed by Puczylowski and Smoktunowicz.
Chebotar, Mikhail, Ke, Wen-Fong, Lee, Pjek-Hwee, Puczylowski, Edmund R.   +3 more
core   +1 more source