Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Mechanics and Failure Mechanisms of Rigid-Flexible 3D-Printed FRP Miura-<i>Ori</i> Structures. [PDF]
Qiao Z +9 more
europepmc +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Triboelectric Nanogenerators in Military: Recent Progress and Critical Challenges. [PDF]
Bao C, Ma X, He M, Yang L, Wang Y.
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Effect of Water-Solid Ratio on the Performance, Microstructure Evolution, and Low-Carbon Characteristics of Multi-Solid-Waste-Based Flowable Stabilized Soil. [PDF]
Ni J, Jiang Q, Zhan Q, Hu H, Zhao Y.
europepmc +1 more source
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Constructing Intrinsically Safe Lithium-Ion Battery Energy Storage via Gradient-Laminated Ceramifiable Silicone Foams. [PDF]
Qiu S, Xu J, Shi C, Zhang L.
europepmc +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Flexible bioelectronic sensors promote the advancement of wearable ultrasound technology in the medicine. [PDF]
Li J +6 more
europepmc +1 more source

