Results 131 to 140 of about 2,755,392 (247)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Spring-mediated cranioplasty for scaphocephaly: techniques and outcomes. [PDF]

Childs Nerv Syst
Guyler MR, Perrault DP, Naidu K, Lopez-Becerra S, Swanson JW, Bartlett SP, Taylor JA, Shakir S.   +7 more
europepmc   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Multi-Center, Real-World Registry Study of the UroLift Prostatic Urethral Lift (PUL) for Benign Prostatic Hyperplasia (BPH) in Japan. [PDF]

Int J Urol
Masumori N, Takahashi S, Anan G, Endo F, Furuta A, Haga K, Hatanaka M, Kato S, Komai Y, Kyoda Y, Matsuyama N, Murayama S, Nagamatsu H, Obinata D, Sekiguchi Z, Waseda Y.   +15 more
europepmc   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Sleep-related breathing disorders beyond infancy following open spina bifida repair. [PDF]

Eur J Pediatr
Lee D, Del Cid-Linares D, Van Speybroeck A, Smith KA, Chmait RH, Keens TG, Ward SLD, Perez IA.   +7 more
europepmc   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Physical activity and glycosylphosphatidylinositol-specific phospholipase D1 (GPLD1) plasma levels in different age cohorts. [PDF]

PLoS One
Raza GS, Jokelainen J, Stenbäck V, Sodum N, Karhu T, Gagnon DD, Leppäluoto J, Järvelin MR, Keinänen-Kiukaanniemi S, Herzig KH.   +9 more
europepmc   +1 more source

The therapeutic opportunities and pitfalls of using iron ion chelators to treat neurodegenerative diseases

FEBS Letters, EarlyView.
Although too much iron in the brain promotes neurodegeneration, iron ion chelators have had mixed effects in clinical trials. This review explains why; some chelators do not render the iron redox‐inactive (e.g., L1) whereas others do (e.g., desferrioxamine).
Barry Halliwell
wiley   +1 more source

Shaping the extinction burst: Increasing its probability and preventing its emergence across topographies. [PDF]

J Exp Anal Behav
Palomino M, Sosa R, Mateos Á, Pellón R, López-Tolsa GE.   +4 more
europepmc   +1 more source