Results 91 to 100 of about 9,914,485 (355)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Impact of Wolf Thresholding on Background Subtraction for Human Motion Detection

Compiler
Series of motion detection based on background subtraction there is an image segmentation stage. Thresholding is a common technique used for the segmentation process. There are two types that can be used in thresholding techniques namely local and global.
Elindra Ambar Pambudi, Muhammad Ivan Nurhidayat   +1 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Jedan pristup detekciji pokreta na slici scene / One approach to movement detection on image

Vojnotehnički Glasnik, 2006
U ovom radu opisan je jedan pristup koji se primenjuje u detekciji pokreta. Naime, koristi se obrada slike razlike formirane oduzimanjem slike pozadine od tekućeg frejma i oduzimanjem sukcesivnih frejmova.
Boban Bondžulić, Žarko Barbarić
doaj   +1 more source

Mini-Open Pedicle Subtraction Osteotomy: Surgical Technique

World Neurosurgery, 2014
Minimally invasive spinal surgery (MISS) has many favorable attributes that would be of great benefit to patients with an adult spinal deformity. Decreased blood loss, lower infection rates, and faster mobilization may help to reduce the high rate of complications associated with these interventions.
Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA ( host institution ), Wang, Michael Y. ( author ), Madhavan, Karthik ( author )   +2 more
openaire   +3 more sources

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Spectroscopy of $B_{c}$ meson in a semi-relativistic quark model using the shifted large-N expansion method

, 2003
We calculate the $c\bar{b}$ mass spectrum, the splitting values and some other properties in the framework of the semi-relativistic equation by applying the shifted large-N expansion technique.
Glkin Y., Kartvelishvili V. G., RAMAZAN SEVER, SAMEER M. IKHDAIR   +3 more
core   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Independently Parameterised Momenta Variables and Monte Carlo IR Subtraction

, 2018
We introduce a system of parameters for the Monte Carlo generation of Lorentz invariant phase space that is particularly well-suited to the treatment of the infrared divergences that occur in the most singular, Born-like configurations of $1\to n$ QCD ...
Cox, Peter, Melia, Tom
core   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source