Upcycled Green Coffee Phenolic-Rich Extract Modulates Key Pathways of Glucose Absorption in Caco-2 Cells: Findings From a Screening of Upcycled Agro-Industrial By-Products for Application in Functional Foods. [PDF]
Mol Nutr Food ResThis study explored the effects of phenolic‐rich extracts from green coffee, orange by‐products, and peanut skin in intestinal carbohydrate metabolism. The extracts were assessed for cytotoxic potential, sucrase‐isomaltase inhibition, glucose transport, and gene expression in a Caco‐2 cell model.
Costa NA +5 more
europepmc +2 more sources
Glycaemic sugar metabolism and the gut microbiota: past, present and future. [PDF]
FEBS JIncreased sugar consumption is a main driver of the increased prevalence of obesity and non‐communicable diseases (NCDs). Therefore, understanding host and microbial sugar metabolism and its metabolic effects is crucial. A high‐sugar diet can induce gut dysbiosis, and gut microbial sugar fermentation can produce deleterious metabolites, potentially ...
Westerbeke FHM +3 more
europepmc +2 more sources
Antidiabetic Potential of Senna siamea: α-Glucosidase Inhibition, Postprandial Blood Glucose Reduction, Toxicity Evaluation, and Molecular Docking. [PDF]
Scientifica (Cairo)Senna siamea (Lam.) H.S. Irwin & Barneby is used in Thai cuisine. This plant is also used in traditional treatments, including diabetes. Therefore, this study aims to examine the antihyperglycemic effects of S. siamea heartwood extract. The ethanolic extract of S. siamea heartwood exhibited activity against α‐glucosidase enzyme with IC50 values of 54.4
Sangkanu S +5 more
europepmc +2 more sources
Pathophysiology of the Neutropenia of GSDIb and G6PC3 Deficiency: Origin, Metabolism and Elimination of 1,5-Anhydroglucitol. [PDF]
J Inherit Metab DisABSTRACT Neutropenia in Glycogen Storage Disease Type Ib (GSDIb) and G6PC3 deficiency results from defects in metabolite repair, leading to the accumulation of 1,5‐anhydroglucitol‐6‐phosphate (1,5‐AG6P). Treatment currently relies on inhibitors of SGLT2, the renal sodium‐glucose co‐transporter, which indirectly enhances urinary excretion of 1,5 ...
Veiga-da-Cunha M +3 more
europepmc +2 more sources
Maturation of Sucrase-Isomaltase Complex in Human Fetal Small and Large Intestine during Gestation [PDF]
Pediatric Research, 1985 Sucrase-isomaltase complex is expressed in human small intestine throughout gestation and in the large intestine between 12 and 30 wk. The molecular form of the enzyme was studied in the brush-border membrane fractions by the immunoblotting method. Before 30 wk of gestation, the enzyme is present only as the high molecular weight prosucrase-isomaltase,
N, Triadou, A, Zweibaum
openaire +2 more sources
Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OM
Chinese Medical Journal, 2016 Background: Lung cancer has become the leading cause of death in many regions. Carcinogenesis is caused by the stepwise accumulation of genetic and chromosomal changes.
Yong-Wu Li +4 more
doaj +1 more source
Hydrolysis of palatinose by rat intestinal sucrase-isomaltase complex
Nippon Eiyo Shokuryo Gakkaishi, 1983 ラット小腸粘膜からスクラーゼ・イソマルターゼ複合体を精製し, パラチノースの水解様式を検討した。1) パラチノースの水解能はスクラーゼ, イソマルターゼ複合体の精製に伴って増大した。2) スクラーゼ・イソマルターゼ複合体によって, パラチノースはイソマルトースと拮抗的に水解され。3) パラチノースの水解能は, イソマルターゼ活性と同様にacarboseによって阻害を受けなかった。それゆえ, パラチノースはスクラーゼ・イソマルターゼ複合体のイソマルターゼ活性部位によって水解されることが明らかにされた。
Toshinao GODA, Norimasa HOSOYA
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Localization of intestinal sucrase-isomaltase complex on the microvillous membrane by electron microscopy using nonlabeled antibodies [PDF]
The Journal of Cell Biology, 1978 Microvillous vesicles isolated from rabbit small intestine showed a trilaminar membrane with a rather smooth surface, which was apparently not affected by papain solubilizing sucrase-isomaltase complex or by trypsin unable to solubilize it. When microvilous vesicles or trysinized ones were incubated with immunoglobulin G against the sucrase-isomaltase ...
Y, Nishi, Y, Takesue
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Subunits of the Small‐Intestinal Sucrase · Isomaltase Complex and Separation of Its Enzymatically Active Isomaltase Moiety [PDF]
European Journal of Biochemistry, 1973 The size and number of subunits of the sucrase · isomaltase complex (mol. wt approximately 220000) from rabbit small intestine were determined by dodecylsulfate‐polyacrylamide gel electrophoresis, gel filtration and fingerprint analysis of tryptic hydrolysates.It was found that two subunits of similar size (estimated mol.
A, Cogoli +6 more
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Purification and Characterization of Rat Intestinal Sucrase-Isomaltase Complex
Eiyo To Shokuryo, 1979 白ネズミ小腸粘膜からスクラーゼ-イソマルターゼ複合体を精製して, その性質について観察し, 他の動物のスクラーゼ-イソマルターゼ複合体と比較検討した。1) 白ネズミ小腸粘膜のS-I複合体をパパインを用いて可溶化し, 硫安分画, セファデックスG-200カラムクロマトグラフィー, DEAEセファデックスA-25カラムクロマトグラフィーを行なうことにより, マルターゼ活性をもつ単一の酵素標品を得た。スクラーゼ活性は140倍, イソマルターゼ活性は96倍に上昇した。2) S-I複合体の分子量は, ポリアクリルアミドゲルディスク電気泳動法により215,000と推定された。3) スクラーゼ, イソマルターゼならびにマルターゼ活性のKm値はそれぞれ18, 4.0, 3.7mMであり, いずれの酵素活性も ...
Mitsuyoshi SASAKI +3 more
openaire +2 more sources