Results 1 to 10 of about 183,614 (281)
Mirror-enhanced super-resolution microscopy [PDF]
Light: Science & Applications, 2016 Axial excitation confinement beyond the diffraction limit is crucial to the development of next-generation, super-resolution microscopy. STimulated Emission Depletion (STED) nanoscopy offers lateral super-resolution using a donut-beam depletion, but its ...
Alonas, E +8 more
core +6 more sources
Super-resolution microscopy of mitochondrial mRNAs [PDF]
Nature CommunicationsMitochondria contain their own DNA (mtDNA) and a dedicated gene expression machinery. As the mitochondrial dimensions are close to the diffraction limit of classical light microscopy, the spatial distribution of mitochondrial proteins and in particular ...
Stefan Stoldt +10 more
doaj +3 more sources
Far-field super-resolution chemical microscopy
Light: Science & Applications, 2023 Far-field chemical microscopy providing molecular electronic or vibrational fingerprint information opens a new window for the study of three-dimensional biological, material, and chemical systems.
Mingwei Tang +4 more
doaj +4 more sources
Super-resolution microscopy demystified [PDF]
Nature Cell Biology, 2019 Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best ...
Schermelleh, L +6 more
openaire +5 more sources
Nature Communications, 2021 The trans-Golgi network (TGN) serves as a platform to sort and transport proteins to their final destinations. Here the authors show that the TGN of Arabidopsis consists of spatially and temporally distinct subregions and propose that these zones may ...
Yutaro Shimizu +12 more
doaj +1 more source
Fluorescent sensors for imaging of interstitial calcium
Nature Communications, 2023 Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the scarcity of methods that allow monitoring in tight inter-cell spaces
Ariel A. Valiente-Gabioud +8 more
doaj +1 more source
Super-Resolution Fluorescence Microscopy [PDF]
Annual Review of Biochemistry, 2009 Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D)
Bo, Huang, Mark, Bates, Xiaowei, Zhuang
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Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy
Advanced Science, 2022 Photobleaching is the permanent loss of fluorescence after extended exposure to light and is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal resolution and observation time.
Jiwoong Kwon +2 more
doaj +1 more source
Super-resolution fluorescence polarization microscopy [PDF]
Journal of Innovative Optical Health Sciences, 2018 Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules.
Karl Zhanghao +4 more
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Super-resolution optical microscopy using cylindrical vector beams
Nanophotonics, 2022 Super-resolution optical microscopy, which gives access to finer details of objects, is highly desired for fields of nanomaterial, nanobiology, nanophotonics, etc.
Liu Min +7 more
doaj +1 more source