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Recent innovations in super-resolution microscopy
Methods, 2015Super-resolution microscopy is no longer a novelty. Many laboratories now have commercial super-resolution instruments, although others are only just becoming aware of its potential. Some groups have implemented their own versions while the field's innovators continue to push back the boundaries.
Alex E, Knight, Michelle, Peckham
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Super-resolution Microscopy for Biological Imaging
2021Studying the ultra-fine structures and functions of the subcellular organelles and exploring the dynamic biological events in depth are the key issues in contemporary biological research. Fluorescence bio-imaging has been used to study cell biology for decades.
Zhigang, Yang +4 more
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Super-Resolution Microscopy of Phloem Proteins
2019Super-resolution microscopy bridges the gap between light and electron microscopy and gives new opportunities for the study of proteins that contribute to phloem function. The established super-resolution techniques are derived from fluorescence microscopy and depend on fluorescent dyes, proteins tagged with GFP variants or fluorochrome-decorated ...
Stanfield, Ryan C., Schulz, Alexander
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Super-resolution microscopy with DNA-PAINT
Nature Protocols, 2017Super-resolution techniques have begun to transform biological and biomedical research by allowing researchers to observe structures well below the classic diffraction limit of light. DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) offers an easy-to-implement approach to localization-based super-resolution microscopy, owing to ...
Schnitzbauer, Joerg +4 more
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2015 International Young Scientists Forum on Applied Physics (YSF), 2015
Conventional optical microscopy is limited by diffraction barrier, so for long time improving of resolution was considered impossible. Recent development in super-resolution imaging increases possibilities in researches of different fields such as medicine, biology, material science etc.
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Conventional optical microscopy is limited by diffraction barrier, so for long time improving of resolution was considered impossible. Recent development in super-resolution imaging increases possibilities in researches of different fields such as medicine, biology, material science etc.
openaire +1 more source
Super-Resolution Imaging by Localization Microscopy
2012Photoactivated localization microscopy (PALM) and the related technique of Stochastic optical reconstruction microscopy (STORM) are super-resolution imaging methods based on the precise localization of single molecules. Instruments based on these techniques are now commercially available and are capable of generating images with lateral resolutions in ...
Dylan M, Owen +3 more
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Super-resolution microscopy of the immunological synapse
Current Opinion in Immunology, 2013Deciphering the spatial organisation of signalling proteins is the key to understanding the mechanisms underlying immune cell activation. Every advance in imaging technology has led to major breakthroughs in unravelling how receptor and signalling proteins are distributed within the plasma membrane and how membrane signalling is integrated with ...
Rossy, Jérémie +3 more
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Super‐Resolution Microscopy: A Comparative Treatment
Current Protocols in Cytometry, 2012AbstractOne of the fundamental limitations of optical microscopy is that of diffraction, or in essence, how small a beam of light can be focused by using an optical lens system. This constraint, or barrier if you will, was theoretically described by Ernst Abbe in 1873 and is roughly equal to half the wavelength of light used to probe the system.
James M, Kasuboski +4 more
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