Results 51 to 60 of about 62,273 (254)

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

Hybrid iterative ptychographic super-resolution microscopy

PhotoniX
By utilizing an array detector to replace the single-element detector in confocal microscopy, Image Scanning Microscopy (ISM) overcomes the inherent trade-off between resolution and signal-to-noise ratio (SNR), thereby enhancing its ability to resolve ...
Yuran Huang, Hanchu Ye, Zitong Ye, Enxing He, Xin Luo, Zhimin Zhang, Xinyuan Jiang, Liang Xu, Yubing Han, Xiang Hao, Yueshu Xu, Cuifang Kuang, Xu Liu   +12 more
doaj   +1 more source

Photochemically Active Dyes for Super-Resolution Microscopy

CHIMIA, 2018
The development of super-resolved optical microscopies has revolutionized the way we visualize cell biology. These techniques strongly rely on the use of photochemically active fluorophores that display changes in their photophysical properties ...
Zacharias Thiel, Pablo Rivera-Fuentes
doaj   +1 more source

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

Paclitaxel induces NM2‐dependent cellular contraction through GEF‐H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells

Molecular Oncology, EarlyView.
Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez, Rafael Pérez‐Díaz, Hugo Ramos‐Solano, Marina Garrido‐Casado, Vanessa C. Talayero, Miguel Vicente‐Manzanares   +5 more
wiley   +1 more source

High-density volumetric super-resolution microscopy

Nature Communications
Volumetric super-resolution microscopy typically encodes the 3D position of single-molecule fluorescence into a 2D image by changing the shape of the point spread function (PSF) as a function of depth. However, the resulting large and complex PSF spatial
Sam Daly, João Ferreira Fernandes, Ezra Bruggeman, Anoushka Handa, Ruby Peters, Sarah Benaissa, Boya Zhang, Joseph S. Beckwith, Edward W. Sanders, Ruth R. Sims, David Klenerman, Simon J. Davis, Kevin O’Holleran, Steven F. Lee   +13 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Super-resolution spectroscopic microscopy via photon localization

Nature Communications, 2016
Photon localization microscopy uses stochastic emission events from fluorescent molecules to enable super-resolution imaging, but spectroscopic information is lost.
Biqin Dong, Luay Almassalha, Ben E. Urban, The-Quyen Nguyen, Satya Khuon, Teng-Leong Chew, Vadim Backman, Cheng Sun, Hao F. Zhang   +8 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source