Results 51 to 60 of about 125,463 (218)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Long-Distance Object Recognition With Image Super Resolution: A Comparative Study

IEEE Access, 2018
Monitor systems are ubiquitously deployed in public areas. However, monitor systems face a major challenge regarding long-distance object recognition. Super-resolution constitutes a popular choice to address this challenge. Since super-resolution methods
Xiaomin Yang, Wei Wu, Kai Liu, Pyoung Won Kim, Arun Kumar Sangaiah, Gwanggil Jeon   +5 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Accelerated CT Sub-pixel Super Resolution

e-Journal of Nondestructive Testing
 The resolution of standard CT scans is dictated by the physical constraints of the detector’s sampling resolution. Super resolution is a class of techniques used to improve the reconstruction resolution beyond the physical limitations of the detector ...
Lucas Determan, Kirk Busche, Pradeep Bhattad   +2 more
doaj   +1 more source

Rapid whole-heart CMR with single volume super-resolution

Journal of Cardiovascular Magnetic Resonance, 2020
Background Three-dimensional, whole heart, balanced steady state free precession (WH-bSSFP) sequences provide delineation of intra-cardiac and vascular anatomy. However, they have long acquisition times.
Jennifer A. Steeden, Michael Quail, Alexander Gotschy, Kristian H. Mortensen, Andreas Hauptmann, Simon Arridge, Rodney Jones, Vivek Muthurangu   +7 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

RudLOV is an optically synchronized cargo transport method revealing unexpected effects of dynasore

EMBO Reports
Live imaging of secretory cargoes is a powerful method for understanding the mechanisms of membrane trafficking. Inducing the synchronous release of cargoes from an organelle is key for enhancing microscopic observation.
Tatsuya Tago, Takumi Ogawa, Yumi Goto, Kiminori Toyooka, Takuro Tojima, Akihiko Nakano, Takunori Satoh, Akiko K Satoh   +7 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Focusing super resolution on the cytoskeleton [version 1; referees: 3 approved]

F1000Research, 2016
Super resolution imaging is becoming an increasingly important tool in the arsenal of methods available to cell biologists. In recognition of its potential, the Nobel Prize for chemistry was awarded to three investigators involved in the development of ...
Eric A. Shelden, Zachary T. Colburn, Jonathan C.R. Jones   +2 more
doaj   +1 more source