Results 231 to 240 of about 8,878,259 (313)

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Exploring the occupational protection potential of an intravenous compounding robot across the cytotoxic drug workflow. [PDF]

Front Public Health
Sun J, Liu B, Li Z, Hu R, Bai S.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Widespread ecosystem effects of an extreme fresh pulse event in a temperate marine ecosystem. [PDF]

Sci Rep
Thorne LH, Nye JA, McSweeney JM, Warren J, Price R, Hirtle N, Meza-Fidalgo J, Menz T, Keiling T, Gallagher K, Napoli C, Hoffman Z, Stepanuk J, Blair H, Cole TVN, Kopelman A, McNamara M, O'Brien O, Redfern J, Carlowicz Lee R, Tortorice P, Escalante M, Ogilvie A, Yang H, Ming L.   +24 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The influence of age on emotion-regulatory strategies, emotional work demands, and health/work outcomes: a systematic PRISMA literature review with narrative synthesis. [PDF]

BMC Psychol
Popucza TZ, Eriksson L, Skytt B, Bjärtå A, Eriksson M.   +4 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Silver Deposition on Thin Films of Symmetric Long-Chain Dialkylimidazolium-Based Ionic Liquids. [PDF]

Molecules
Alves ACPM, Santos LMNBF, Costa JCS.
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Research on length deformation control of high-altitude linear engineering based on the ellipsoid expansion method. [PDF]

Sci Rep
Yang W, He J, Ding X, Li F, Qian L, Gan L, Wei K.   +6 more
europepmc   +1 more source