Results 91 to 100 of about 100,663 (270)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Revisiting sylvian fissure dissection - A preliminary investigation into surgical process modelling for evaluating surgical proficiency

Brain and Spine
Introduction: Understanding the factors that contribute to efficient surgical behaviour and the prevention of technical errors poses a significant challenge in neurosurgery. Current training curricula lack proficiency-centred training and objective tools
Félix Buyck, Jef Vandemeulebroucke, Ine Dirks, Jan Frederick Cornelius, Johnny Duerinck, Sebastien Froelich, Henry Schroeder, Wietse Geens, Frederick Van Gestel, Michaël Bruneau   +9 more
doaj   +1 more source

An analysis on the effect of body tissues and surgical tools on workflow recognition in first person surgical videos

International Journal of Computer Assisted Radiology and Surgery
Abstract Purpose Analysis of operative fields is expected to aid in estimating procedural workflow and evaluating surgeons’ procedural skills by considering the temporal transitions during the progression of the surgery.
Hisako Tomita, Naoto Ienaga, Hiroki Kajita, Tetsu Hayashida, Maki Sugimoto   +4 more
openaire   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Reducing Medication Errors Through Workflow Redesign [PDF]

, 2017
Lack of medication reconciliation at the point of transitions between skilled nursing facilities/nursing homes (SNF/NHs) and acute care hospitals (ACHs) is a common point of origin for medical errors that cause harm to patients.
Blake, Rosslyn W, DNP, APRN, GNP
core   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Cone-beam computed tomography-guided volumetric assessment of secondary alveolar bone grafting

Scientific Reports
This study aimed to develop and implement a standardized 3D workflow for surgical planning and outcome assessment in secondary alveolar bone grafting (SABG) using cone-beam computed tomography (CBCT). A 3D protocol was developed using medically certified
Julian A. Erkert, Benito K. Benitez, Yoriko Lill, Sebastian Tapia Coron, Andreas A. Mueller   +4 more
doaj   +1 more source

Using Business Modeling to Streamline Cost of Anesthesia in a Cardiopulmonary Laboratory [PDF]

, 2017
The Time Driven Activity Based Costing (TDABC) measurement system, a model used in the business arena, can capture clinical processes and costs that other costing models often overlook.
Dang, Johnny, DNP, CRNA, Starnes-Ott, Kristen, PhD, CRNA   +1 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Cataract-1K Dataset for Deep-Learning-Assisted Analysis of Cataract Surgery Videos

Scientific Data
In recent years, the landscape of computer-assisted interventions and post-operative surgical video analysis has been dramatically reshaped by deep-learning techniques, resulting in significant advancements in surgeons’ skills, operation room management,
Negin Ghamsarian, Yosuf El-Shabrawi, Sahar Nasirihaghighi, Doris Putzgruber-Adamitsch, Martin Zinkernagel, Sebastian Wolf, Klaus Schoeffmann, Raphael Sznitman   +7 more
doaj   +1 more source