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Colonization of aposymbiotic Aiptasia with Symbiodinium v1
This protocol describes briefly how to perform colonization of aposymbiotic Aiptasia with Symbiodinium cultures. The method isbased on the one described in Xiang et al., 2013.
Grossman Lab, Pringle Lab
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Expanding the population genetic perspective of cnidarian‐Symbiodinium symbioses [PDF]
The modern synthesis was a seminal period in the biological sciences, establishing many of the core principles of evolutionary biology that we know today. Significant catalysts were the contributions of R.A. Fisher, J.B.S. Haldane and Sewall Wright (and others) developing the theoretical underpinning of population genetics, thus demonstrating adaptive ...
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Coral bleaching events are increasing in frequency, demanding examination of the physiological and molecular responses of scleractinian corals and their algal symbionts (Symbiodinium sp.) to stressors associated with bleaching.
Sarah W. Davies +5 more
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Marine Invertebrate Larvae Associated with Symbiodinium: A Mutualism from the Start?
Symbiodinium are dinoflagellate photosynthetic algae that associate with a diverse array of marine invertebrates, and these relationships are comprehensively documented for adult animal hosts.
Miguel Mies +3 more
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Symbiodinium-enriched RNA extraction from Aiptasia holobiont v1 [PDF]
This protocol enriches holobiontExaiptasia pallida tissue forSymbiodinium, removing more than 99% of host tissue while maintaining high RNA integrity (RIN > 7). The symbiont-enriched fraction is subsequently lysed via bead beating and rna isolated using the Qiagen RNeasy Mini kit.
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Fluorescence from a single Symbiodinium cell
The partnership between coral and its algal symbionts, Symbiodinium, is crucial to the global environment. Yet, the regulatory process within the photosynthetic machinery of Symbiodinium is still not clearly understood. Here, we studied the influence of light stress from focussed red and blue lasers on single Symbiodinium cells.
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Total RNA Extraction from Dinoflagellate Symbiodinium Cells
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Microsatellite loci for Symbiodinium goreaui and other Clade C Symbiodinium
Conservation Genetics Resources, 2013The genus Symbiodinium comprises a diverse group of dinoflagellates known for their obligate relationship with reef–building corals. Members of the sub-genus ‘clade C’ are abundant, geographically wide-spread, as well as genetically and ecologically diverse. Coral colonies harboring clade C are often the most exposed to physical stressors.
Drew C. Wham +2 more
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Conservation Genetics Resources, 2011
Nine new polymorphic microsatellites were developed for Symbiodinium trenchi (sensu type D1a). These loci were tested on populations of S. trenchi from corals in Palau and 3–19 alleles were observed at each haploid locus with an average of 7 alleles.
Drew C. Wham +2 more
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Nine new polymorphic microsatellites were developed for Symbiodinium trenchi (sensu type D1a). These loci were tested on populations of S. trenchi from corals in Palau and 3–19 alleles were observed at each haploid locus with an average of 7 alleles.
Drew C. Wham +2 more
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Recent progress in Symbiodinium transcriptomics
Journal of Experimental Marine Biology and Ecology, 2011Abstract Dinoflagellate symbionts of the genus Symbiodinium are integral to the success of the coral holobiont (a coral host and the microbial community it harbours), however despite their importance we currently have a very limited knowledge of the genes which they possess and their genomic organisation.
Leggat, William +2 more
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