Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Synchronized rotor angle measurement of synchronous machines [PDF]
A key input parameter to governor feedback control and stability protection of generators is the angle of the induced voltage internal to the generator. Current practice is to estimate this value using measurements from the terminals of the generator and mathematical models. This project aims to develop a system that would directly measure the internal
Jaime De La Ree, David C. Mazur
openaire +1 more source
STUDY OF TRANSIENT AND STATIONARY OPERATION MODES OF SYNCHRONOUS SYSTEM CONSISTING IN TWO MACHINES
The solution of the problem of reliable functioning of an electric power system (EPS) in steady-state and transient regimes, prevention of EPS transition into asynchronous regime, maintenance and restoration of stability of post-emergency processes is ...
V. S. Safaryan
doaj +1 more source
A Two‐Stage Questionnaire and Actigraphy Screening for iRBD in a Multicenter Retrospective Cohort
ABSTRACT Objective Isolated rapid‐eye‐movement sleep behavior disorder is a prodromal marker of synucleinopathies. However, most cases remain undiagnosed due to the insufficient predictive value of questionnaires and limited access to confirmatory video‐polysomnography. We assessed a two‐stage screening strategy combining a brief questionnaire on rapid‐
Caleb A. Massimi +17 more
wiley +1 more source
Evaluating the (q-d) Axis Parameters of Three Phase Salient Pole Synchronous Machine
In order to perform any simulated work like using MATLAB Simulink for a salient pole synchronous machine the direct-axis (d-axis) synchronous reactance xd and quadrature-axis (q-axis) synchronous reactance xq ((q-d) axis) and other parameters must be ...
Omar T. Mahmoud
doaj +1 more source
Synchronous machine parameter identification in frequency and time domain [PDF]
This paper presents the results of a frequency and time-domain identification procedure to estimate the linear parameters of a salient-pole synchronous machine at standstill.
Hasni M. +5 more
doaj +1 more source
Standardization tests for the industrialization of grid-friendly Virtual Synchronous Generators [PDF]
Three synchronous machine models representing three precision levels (complete, reduced and static), implemented in a virtual synchronous generator (VSG)-based industrial inverter, are compared and discussed to propose a set of tests for a possible ...
V. Moulichon +5 more
doaj +1 more source
A Q‐Learning Algorithm to Solve the Two‐Player Zero‐Sum Game Problem for Nonlinear Systems
A Q‐learning algorithm to solve the two‐player zero‐sum game problem for nonlinear systems. ABSTRACT This paper deals with the two‐player zero‐sum game problem, which is a bounded L2$$ {L}_2 $$‐gain robust control problem. Finding an analytical solution to the complex Hamilton‐Jacobi‐Issacs (HJI) equation is a challenging task.
Afreen Islam +2 more
wiley +1 more source

