Results 31 to 40 of about 382,124 (263)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Examining unsafe injection practices associated with auto-disable (AD) syringes: a systematic review

Human Vaccines & Immunotherapeutics, 2021
Auto-disable (AD) syringes are specifically designed to prevent syringe reuse. However, the notion that specific AD syringe designs may be unsafe due to reuse concerns related to the syringe’s activation point has surfaced.
Anokhi Ali Khan, Mehr Munir, Fatima Miraj, Shayan Imran, Danya Arif Siddiqi, Arshad Altaf, Aamir Javed Khan, Subhash Chandir   +7 more
doaj   +1 more source

ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function

FEBS Open Bio, EarlyView.
Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley, Luke W. Molesworth, Nethma H. Waduge, Fahimeh Eskandari, Peter R. Boag, Gregory M. Davis   +5 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Stability and compatibility of phentolamine mesylate combined with furosemide in common infusion solutions for pediatric critical care

Frontiers in Pediatrics
BackgroundIntravenous phentolamine mesylate and furosemide are widely administered via infusion pumps to treat severe pediatric critical illnesses. Their off-label concurrent use lacks reliable physicochemical compatibility and stability data, bringing ...
Baoxia Fang, Ruiling Shang, Fuchao Chen, Yanhong Liu, Huimin Liu   +4 more
doaj   +1 more source

IMPROVEMENTS IN SYRINGES. [PDF]

The Lancet, 1884
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openaire   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The effect of mepolizumab dosage form on treatment outcomes in severe asthma

Frontiers in Medicine
BackgroundA monoclonal antibody such as mepolizumab typically first appears as a parenteral lyophilized formulation (LYO), then as various parenteral solution forms, and finally as a self-administered form at homecare.
Martina Vetchá, Kateřina Kubová, Constantinos Glynos, Sylvie Pavloková, Irena Krčmová, Irena Krčmová, Eva Voláková, Eva Voláková, Ondřej Fibigr, Ondřej Fibigr, Beáta Hutyrová, Beáta Hutyrová, Alena Vlachová, Jiří Zeman, David Vetchý   +14 more
doaj   +1 more source

Impact of an air bubble within the syringe on test results obtained with a modern blood gas analyzer [PDF]

Journal of Medical Biochemistry
Background: Minimizing air aspiration by carefully filling blood gas syringes is crucial to prevent air contamination from causing undesirable variations in gasses and other molecules.
Pighi Laura, Salvagno Gian Luca, Ferraro Roberta, Celegon Giovanni, Henry Brandon M., Lippi Giuseppe   +5 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source