Results 231 to 240 of about 24,523,468 (288)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Application Usability, Engagement, and Postpartum Weight Retention: Secondary Analysis of the INTER-ACT Randomized Controlled Trial. [PDF]

Mayo Clin Proc Digit Health
Duizer L, Geusens F, Geerits E, Devlieger R, Bogaerts A.   +4 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Spanish adaptation and validation of the Capability Cards for use in community primary health care. [PDF]

Prim Health Care Res Dev
Llubes-Arrià L, Boonekamp G, Brummel A, Sol-Culleré J, Mateos JT, Salanterä S.   +5 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Co-Designing a Clinician-Centered Video Gait Assessment System for Cerebral Palsy Based on the Framework for Co-Design of Clinical Practice Tools: Iterative Development and Formative Evaluation Study. [PDF]

J Med Internet Res
Gao X, Cheng X, Sun J, Liang W, Reading S, Zhang Y.   +5 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Pictorial System Usability Scale (P-SUS)

Baumgartner, Juergen, Frei, Naomi, Kleinke, Mascha, Sauer, Juergen, Sonderegger, Andreas   +4 more
openaire   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Development and Preliminary Evaluation of the OCODES Digital Strategy in Response to Health Misinformation: Nominal Group Technique and Retrospective Usability Evaluation Study. [PDF]

JMIR Form Res
Echániz-Serrano E, Ramón-Arbués E, Granada-López JM, Fernández-Rodrigo MT, Satústegui-Dordá PJ, Benito-Ruiz E, Samaniego-Díaz de Corcuera ME, Barrio-Forné N, Gómez-Torres P.   +8 more
europepmc   +1 more source