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Protocol to Identify Unknown Flanking DNA Using Partially Overlapping Primer-based PCR for Genome Walking. [PDF]
Bio ProtocJia M, Ding D, Liu X, Li H.
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Identification and elimination of DNA sequences in Taq DNA polymerase [PDF]
Journal of Clinical Microbiology, 1994 This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.
Michael S. Hughes +2 more
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Structure of Taq polymerase with DNA at the polymerase active site
Nature, 1996The DNA polymerase from Thermus aquaticus (Taq polymerase) is homologous to Escherichia coli DNA polymerase I (Pol I) and likewise has domains responsible for DNA polymerase and 5' nuclease activities. The structures to the polymerase domains of Taq polymerase and of the Klenow fragment (KF) of Pol I are almost identical, whereas the structure of a ...
Jimin Wang +3 more
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NaOH Treatment to Neutralize Inhibitors of Taq Polymerase
Journal of Forensic Sciences, 1999Abstract The introduction of polymerase chain reaction (PCR) into the forensic field has greatly extended the ability to analyze DNA from small or degraded samples. However, one significant problem with PCR analysis is the sensitivity of Taq Polymerase to inhibitors found in many substrates commonly encountered with evidentiary materials.
Carol A. Scherczinger +3 more
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Springer Reference Medizin, 1989
The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E.
D. Gelfand
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The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E.
D. Gelfand
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Taq DNA Polymerase Mutants and 2′-Modified Sugar Recognition
Biochemistry, 2015Chemical modifications to DNA, such as 2' modifications, are expected to increase the biotechnological utility of DNA; however, these modified forms of DNA are limited by their inability to be effectively synthesized by DNA polymerase enzymes.
Aaron M. Leconte +9 more
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Inhibition of Taq polymerase as a method for screening heparin for oversulfated contaminants
Biomaterials, 2008Heparin and low molecular heparins are extensively used in the treatment of a wide range of diseases in addition to their classic anticoagulant activity and can be found coating medical devices such as catheters, stents and filters. Early in 2008, a sharp increase in heparin-associated severe adverse events, including over 80 deaths, was linked to the ...
Cecilia Tami +6 more
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Primer/Template-Independent Synthesis of Poly d(A-T) by Taq Polymerase
Biochemical and Biophysical Research Communications, 1997Taq DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C. Tth DNA polymerase also catalyzed the reaction, while delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not.
Kenji Yamamoto +8 more
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A-Tailing with Taq Polymerase v3
, 2020This protocol can be used to add As to the blunt-ends of DNA fragments that have been amplified using a high-fidelity polymerase (such as Q5® High Fidelity DNA Polymerase).
New England Biolabs
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