Results 311 to 320 of about 819,635 (326)

Genomic Sequencing with Taq Polymerase (Linear Amplification)

1990
The use of Taq polymerase for the linear amplification of genomic DNA fragments by chemical sequencing reactions (Saluz and Jost, 1989) retains all the benefits of the classical procedure (Church and Gilbert, 1984), while being radically simplified. The scheme of this procedure is shown in the flow diagram (Fig. IV. 1).
J. P. Jost, H. P. Saluz
openaire   +2 more sources

Study on the Interaction of Colloidal Gold with Taq DNA Polymerase

Chinese Journal of Chemistry, 2007
Abstract The interaction of colloidal gold with Taq DNA polymerase (Taq) was investigated in this study. Taq‐gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV‐Vis spectroscopy, X‐ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized
Hongping Zhu, Wenfeng Wang, Li-Juan Mi, SD（姚思德） Yao, Shimou Chen   +4 more
openaire   +2 more sources

Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification

Protein & Peptide Letters, 2007
We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the "long PCR" up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a "long buffer." We propose that the most important requirements are the survival rate ...
Young Kee Chae, Kyung-Nam Kim, Hayan Lee
openaire   +3 more sources

A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli

Archives of Microbiology, 2020
R. Din, Muhammad Islam Khan, A. Jan, Shahzad Akbar Khan, Irshad Ali   +4 more
semanticscholar   +1 more source

High-Efficiency Separation and Purification of Taq DNA Polymerase

2017
Taq DNA polymerase is a thermostable DNA polymerase with high specificity and efficiency for DNA amplification and it is widely used in PCR. In this pap/er, we explored a method of isolation and purification of Taq DNA polymerase from a strain of our lab.
Xiangchao Gu, Hao Zhou, Ai Mu, Zhiyin Hu, Yujie Zhang   +4 more
openaire   +2 more sources

New rulings on the cetus patent for taq polymerase

Molecular Diagnosis, 2000
D G, Leonard, R S, Schifreen, J J, Sninsky, T J, White   +3 more
openaire   +3 more sources

Cinnamic Acid Derivatives Acting against Aspergillus Fungi.Taq Polymerase I a Potential Molecular Target

Natural Product Communications, 2012
Lucas J. Gutiérrez, M. L. Mascotti, M. Kurina-Sanz, C. Pungitore, R. Enriz, F. Giannini   +5 more
semanticscholar   +1 more source

Availability of Taq polymerase

Science, 1993
openaire   +3 more sources

Detection of mismatched caspase-3 DNA oligonucleotides with an SPR biosensor following amplification by Taq polymerase

Microchimica Acta, 2012
Ying Zhang, Ying Mu, Chao Zhou, Q. Song, W. Jin, Q. Jin   +5 more
semanticscholar   +1 more source

Micro- and nanoparticles of condensed DNA Formed in PCR with Taq polymerase and plasmid DNA as a template

Microbiology, 2011
V. N. Danilevich, E. A. Vasilenko, E. Pechnikova, O. Sokolova, E. Grishin   +4 more
semanticscholar   +1 more source