Results 231 to 240 of about 262,135 (326)
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Correction: Comprehensive study of rapid capacity fade in prismatic Li-ion cells with flexible Packaging. [PDF]
Sci RepStravova Z +7 more
europepmc +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Experimental study on axial compression performance of I-shaped aluminum concrete columns embedded in CFRP-PVC square tubes. [PDF]
PLoS OneWang Y, Wang M, Tang C, Yu Y, Pang J.
europepmc +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Elderly Man With Severe Eye Pain After Practicing Eye Drop Technique in Dim Light. [PDF]
J Am Coll Emerg Physicians OpenFukuoka H, Sotozono C.
europepmc +1 more source
FEBS Open Bio, EarlyView.The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan +6 more
wiley +1 more source
Semiembedded HDPE/VIS Composite Membranes with Tailored Wettability and Superior Mechanical Durability for Outdoor Protective Applications. [PDF]
ACS OmegaZhang H, Zhai Q, Zhen Q, Lv H, Ye K.
europepmc +1 more source