Results 71 to 80 of about 909,554 (261)

Test methods for specific heat capacity of frozen soil based on principles of mixing calorimetry

open access: yesYantu gongcheng xuebao, 2018
The specific heat capacity of soil is an important parameter in the ground freezing method. However, the specific heat capacity obtained by the exiting mixed calorimetric methods is the average specific heat capacity from a negative temperature to the ...
LI Shun-qun 1, 2, WANG Xing-xing 1, 2, XIA Jin-hong 3, SHEN Dao-ming 3
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Comparative Analysis of the Influence of Different Preparation Methods on the Properties of TEMPO-Oxidized Bacterial Cellulose Powder Films

open access: yesJournal of Natural Fibers
The preparation method of cellulose films plays a vital role in their properties. This work aims to characterize 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized bacterial cellulose powder (TOBCP) films prepared with three different methods ...
Dieter Rahmadiawan   +8 more
doaj   +1 more source

Effect of wall heat insulation on wall heat transfer mechanism in rapid compression and expansion environment

open access: yesNihon Kikai Gakkai ronbunshu, 2018
Heat insulation by a wall material which has low heat capacity and heat conductivity is one of the effective ways to reduce heat transfer. By the heat insulated coating, wall temperature is fluctuated following the behavior of in-cylinder gas temperature,
Yuji HARADA   +5 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Development and Validation of A Critical Thinking Assessment on Temperature and Heat for Secondary Physics Education

open access: yesEducational Process: International Journal
Background/purpose. Critical thinking (CT) is fundamental in science education, but instruments to measure CT in specific domains, such as physics, are still limited.
Bangun Sartono   +3 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

open access: yesFEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer   +7 more
wiley   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

open access: yesFEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento   +6 more
wiley   +1 more source

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