Results 121 to 130 of about 1,043,977 (317)
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Cifra: Компьютерные науки и информатикаРассмотрен подход к многофакторной аутентификации с использованием биометрии и токена. Предложена методика формирования паролей для аутентификации из нескольких компонентов, один из которых размещается в защищенном хранилище токена и становится доступным
Беломойцев Д.Е. +1 more
doaj +1 more source
, 2020 Writing process template for journal articles and other written works produced within the context of the Strengthening Knowledge Management for Greater Development Effectiveness (SKIM ...
Vansant, Emilie
core
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
, 2019 This template is used to consolidate the list of activities per status (completed in closing year, new and/or ongoing in incoming year) for the CRP ...
Bonaiuti, Enrico
core
ICARDA Indicators Performance Tracking Table (IPTT) Template
, 2022 The IPTT template is an integral part of the ICARDA MEL framework. If used consistently, it will enhance the understanding of project performance across the institution.
Ndoro, Maurice
core
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
W3/Bil GLDC Mapping template 2020
, 2019 This project mapping template is used by the CRP-GLDC to consolidate details of projects per status (ending in the closing year, new or ongoing in the incoming year), filled at the institute level. Note: A separate mapping approval template is to be used
Bonaiuti, Enrico
core
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source