FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
TED1 Promotes the Assembly of SCF<sup>D3</sup>-D14-D53 to Reinforce Strigolactone Signaling in Rice. [PDF]
Adv Sci (Weinh)Luo S +22 more
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
The <i>C</i>-Terminal Phenylalanine of the Thanatin-like Peptide Rip-2 Contributes to Its Enhanced LptA-Associated Antimicrobial Activity Compared with Thanatin. [PDF]
Int J Mol SciPanteleev PV +8 more
europepmc +1 more source
Synthesis of Control of Terminal Acceleration of Spatial Rotations [PDF]
Among the problems of Adaptive Terminal Control, one of the most important is the problem of control of acceleration process of moving objects. Two cases are discussed: Control with arbitrary terminal acceleration and control with certain value of ...
Cabir Ergüven
core
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The Role of Terminal Heavy Chalcogenide Ligands in Enabling Hydride Transfer Reactivity of W═E (E = S, Se) Models for Molybdenum Hydroxylases. [PDF]
J Am Chem SocModappilappally AV, Mankad NP.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Structural Characterization and Engineering of a GH134 β-Mannanase from Aspergillus nidulans for Enhancement of Activity and Stability. [PDF]
J Agric Food ChemChen SC +8 more
europepmc +1 more source