Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Presidents Circle, University of Utah [04]
Photo shows the top of what became known as Presidents Circle, with administration (Park) building to the ...
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Are all digital hoarding behavior alike? Uncovering the pathways to digital hoarding behavior in the workplace. [PDF]
Dong Z, Bao M.
europepmc +1 more source
Circle-packings and circle-coverings on a cylinder.
Bleicher, M. N., Fejes Tóth, L.
openaire +3 more sources
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Chautauqua Circle Members, September 21, 1998
Marylin Holmes, Andretta Nance, Sylvia Suitt, and Alexis Scott stand in the corner of a room. Written on verso: Chautauqua Circle New Members, Marylin Holmes, Andretta Nance (Saxon), Sylvia Suitt, M.
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Laparoscopic intracavitary catheterization combined with bleomycin quadruple sclerotherapy for retroperitoneal lymphatic malformations: a single-center case series. [PDF]
Zhang G +7 more
europepmc +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Imaging of retinal nerve fiber in non-arteritic anterior ischemic optic neuropathy at acute, sub-acute, and chronic phases: adaptive optics scanning laser ophthalmoscopy versus optical coherence tomography. [PDF]
Gong Y +5 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source

