Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
A Unified Method for Detecting Phylogenetic Signals in Continuous, Discrete, and Multiple Trait Combinations. [PDF]
Ecol EvolYao L, Yuan Y.
europepmc +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
, 1986 University of Minnesota. Special Committee on Unified and Increased Preparation Requirements, 1986. (1986). Unified and Increased Preparation Requirements, Special Committee Final Report, March 26, 1986. Retrieved from the University Digital Conservancy,
University of Minnesota. Special Committee on Unified and Increased Preparation Requirements, 1986
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Retraction notice to "Application of the unified method to solve the ion sound and Langmuir waves model" [Heliyon 8 (2022) e10924]. [PDF]
HeliyonNandi DC, Ullah MS, Roshid HO, Ali MZ.
europepmc +1 more source
FTIR spectroscopy as a unified method for simultaneous analysis of intra- and extracellular metabolites in high-throughput screening of microbial bioprocesses. [PDF]
Microb Cell Fact, 2017 Kosa G +3 more
europepmc +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Electro-deformation spectroscopy: A unified method for simultaneous electrical and mechanical characterization of single cells. [PDF]
Acta BiomaterDu E, Xu H, Ponkratova L.
europepmc +1 more source
Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers
FEBS Letters, EarlyView.Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui +4 more
wiley +1 more source
In vivo validation of the unified barge method to assess the bioaccessibility of metals in soils
, 2011 International audienceThe bioaccessibility research group of Europe (BARGE) has set up a European unified method (Unified Barge Method, UBM) to assess the oral bioaccessibility of potentially harmful elements (PHEs) in soils. This research focuses on the
Wragg, Joanna +6 more
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