Results 231 to 240 of about 11,003 (249)
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Organoid culture of mouse fallopian tube epithelial stem cells with a thermo-reversible gelation polymer

Tissue and Cell, 2021
In this study, a three-dimensional (3D) thermo-reversible gelation polymer (TGP) culture system was established for organoid culture of mouse fallopian tube (FT) epithelial stem cells (FTESCs) without cell isolation. FT tissues from 6- to 8-week-old ICR mice were digested with collagenase, and whole FT cells (FTCs) were inoculated into the TGP. After 6
Fan Lin
exaly   +3 more sources

A New Method to Prepare Multicellular Spheroids in Cancer Cell Lines Using a Thermo-Reversible Gelation Polymer

Artificial Organs, 2003
Abstract: The purpose of this study is to utilize the thermo‐reversible gelation polymer in which the sol‐gel transitting phase is reversibly changed by temperature in a three‐dimensional culture system. Human cancer cells have been observed to form multicellular spheroids, whereas fibroblasts slowly develop into small spheroids with the culture medium
Yuko Kurahashi   +2 more
exaly   +3 more sources

Microfluidic cell sorter with flow switching triggered by a sol–gel transition of a thermo-reversible gelation polymer

Sensors and Actuators B: Chemical, 2010
Abstract This paper presents a new strategy for sorting fluorescently labeled cells with a microfluidic device. Cell separation was performed with flow switching using changes in fluidic resistance in a waste channel caused by sol–gel transition of a thermo-reversible gelation polymer (TGP).
Hirokazu Sugino   +2 more
exaly   +2 more sources

A New Chemosensitivity Test Using a Thermo-Reversible Gelation Polymer for Recurrent Gynecologic Cancer Patients and a Preliminary Study of Mechanisms of Anticancer Drug Resistance

Human Cell, 2008
TGP (thermo-reversible gelation polymer) is a high molecular compound that has so-gel transmitting temperature of 221C Since solid cancer tissue grows in this polymer three-dimensionally, and fibroblasts scarcely grow in it, TGP is suitable for chemosensitivity assays for solid tumors.
Yoichi Kobayashi   +2 more
exaly   +3 more sources

A Novel Three-Dimensional Culture System for Isolation and Clonal Propagation of Neural Stem Cells Using a Thermo-Reversible Gelation Polymer

Tissue Engineering - Part C: Methods, 2009
In the present study, we examined the possible utility of a three-dimensional culture system using a thermo-reversible gelation polymer to isolate and expand neural stem cells (NSCs). The polymer is a synthetic biologically inert polymer and gelates at temperatures higher than the gel-sol transition point ( approximately 20 degrees C). When fetal mouse
Reinhold J Medina   +2 more
exaly   +4 more sources

Periodontal regeneration by autologous bone marrow mononuclear cells embedded in a novel thermo reversible gelation polymer.

Journal of stem cells, 2014
Regeneration of bony defects caused by periodontal disease continues to be a challenge for clinicians. Application of stem cells from different tissue sources and scaffolds for regeneration have been reported in animal models but clinical studies with long term follow-ups are limited.
Seshadri, Sankaranarayanan   +4 more
openaire   +1 more source

Thermo-reversible gelation of myofibrillar protein: Relationship between coiled-coil and thermal reversibility

Current Research in Food Science, 2023
Lingying Zhang   +2 more
exaly  

Effect on the Thermo‐Gelation Process of the Degree and Molar Substitution of HPMC Polymer Hydrogels

Macromolecular Symposia, 2022
Saray Pérez-Robles   +2 more
exaly  

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