Results 111 to 120 of about 7,342 (228)

16 S rDNA primers and the unbiased assessment of thermophile diversity

open access: yes, 2004
Our understanding of thermophile diversity is based predominantly on PCR studies of community DNA. ‘Universal’ and domain-specific rRNA gene PCR primers have historically been used for the assessment of microbial diversity without adequate regard to ...
Baker, Gillian, Cowan, Donald A.
core  

Structural Properties of Ribosomal Protein S8 from the Extreme Thermophile Thermus Thermophilus

open access: yes, 1995
The gene of ribosomal protein S8 from the extreme thermophile Thermus thermophilus was expressed in E. coli using the strain BL21(DE3) and vector pET3-1.
Uversky, Vladimir N.   +5 more
core   +1 more source

Leucyl-tRNA synthetase from the extreme thermophile Aquifex aeolicus has a heterodimeric quaternary structure

open access: yes, 2002
Class I aminoacyl-tRNA synthetases have been thought to be single polypeptide enzymes. However, the complete genome sequence of a hyper thermophile Aquifex aeolicus suggests that the gene for leucyl-tRNA synthetases (LeuRS) is probably split into two ...
Gouda, Masaki   +7 more
core   +1 more source

Optimization of xylanase production by Sporotrichum thermophile using corn cobs and response surface methodology

open access: yes, 2006
A 32 central composite experimental design was performed with the aim of optimizing the production of xylanase by Sporotrichum thermophile grown on corn cobs in submerged cultures.
Katapodis, P   +5 more
core   +1 more source

Combined computational, rational, and empirical design of boiling-resistant keratinase

open access: yesApplied and Environmental Microbiology
Engineering of highly thermostable keratinase is of great theoretical interest in understanding protein stability mechanisms and practical significance for processing keratinous wastes such as feathers and wool.
Yong Yang   +10 more
doaj   +1 more source

Bioprocess exploration for thermostable α-amylase production of a deep-sea thermophile Geobacillus sp. in high-temperature bioreactor

open access: yes, 2016
Geobacillus sp. 4j, a deep-sea high-salt thermophile, was found to produce thermostable α-amylase. In this work, culture medium and conditions were first optimized to enhance the production of thermostable α-amylase by statistical methodologies.
Xiangshan Zhou (837935)   +6 more
core   +1 more source

Genome mapping of an extreme thermophile, Thermus caldophilus GK24

open access: yes, 2003
Genome of an extreme thermophile, Thermus caldophilus GK24 has been analyzed to construct the genomic map. The genomic DNAs encapsulated in agarose gel were digested with SspI, EcoRI, SpeI, and HpaI restriction endonucleases, and then the resulting ...
김명희
core   +1 more source

Corrigendum: Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production

open access: yesFrontiers in Microbiology, 2019
Marta Irla   +8 more
doaj   +1 more source

PRODUCTION OF LIPASE BY SPOROTRICHUM (CHRYSOSPORIUM) THERMOPHILE APINIS IMMOBILIZED IN ALGINATE AND POROUS GLASS BEADS

open access: yes
Spores and mycelia of Sporotrichum (Chrysosporium) thermophile Apinis were immobilized in alginate and porous glass beads for lipase production in batch replacement culture and a column reactor.
Klein, J.   +3 more
core  

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