Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Green synthesis of porous carbon guided by phase diagrams for electrochemical rubidium salt desalination. [PDF]
Li B +8 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Milling Stability Prediction Considering Axial Geometric Contact Effects. [PDF]
Zhang Y, Ren X, Yang J.
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This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
CaMKIIα mediates feedback effects of wheel-running activity on the circadian pacemaker in mice. [PDF]
Yoshikawa T +3 more
europepmc +1 more source
Identifying key outcomes for social prescribing research in the US: a Delphi method study. [PDF]
Sonke J, Morgan N, Fancourt D.
europepmc +1 more source
A Hand-Based Method for Calculating Microemulsion Phase Equilibrium in Chemical Enhanced Oil Recovery. [PDF]
Pang C, Zhang Y, Zhu L, Zhang M.
europepmc +1 more source
Real-world energy data of 200 feeders from low-voltage grids with metadata in Germany over two years. [PDF]
Treutlein M +7 more
europepmc +1 more source
Optimization of Extraction Process for Total Flavonoids and Crude Polysaccharides from <i>Nephrolepis cordifolia</i> and Assessment of Their In Vitro Antioxidant Properties. [PDF]
Zhong Y +6 more
europepmc +1 more source

