Time-resolved studies define the nature of toxic IAPP intermediates, providing insight for anti-amyloidosis therapeutics [PDF]
eLife, 2016 Islet amyloidosis by IAPP contributes to pancreatic β-cell death in diabetes, but the nature of toxic IAPP species remains elusive. Using concurrent time-resolved biophysical and biological measurements, we define the toxic species produced during IAPP ...
Andisheh Abedini +15 more
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Opportunities and challenges for time-resolved studies of protein structural dynamics at X-ray free-electron lasers. [PDF]
Philos Trans R Soc Lond B Biol Sci, 2014 X-ray free-electron lasers (XFELs) are revolutionary X-ray sources. Their time structure, providing X-ray pulses of a few tens of femtoseconds in duration; and their extreme peak brilliance, delivering approximately 1012 X-ray photons per pulse and ...
Neutze R.
europepmc +2 more sources
Time-resolved studies with FELs [PDF]
Journal of Electron Spectroscopy and Related Phenomena, 2015 Abstract Intense femtosecond VUV, XUV, and X-ray pulses from free-electron lasers (FELs) enable time-resolved experiments studying ultrafast dynamics in a large variety of systems relevant, e.g., to physics, chemistry, biology, and material sciences.
Rudenko , A., Rolles, D.
openaire +6 more sources
Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection [PDF]
IUCrJ, 2021 Time-resolved crystallography of biomolecules in action has advanced rapidly as methods for serial crystallography have improved, but the large number of crystals and the complex experimental infrastructure that are required remain serious obstacles to ...
Jonathan A. Clinger +4 more
doaj +2 more sources
A cryo-EM grid preparation device for time-resolved structural studies
IUCrJ, 2019 Structural biology generally provides static snapshots of protein conformations that can provide information on the functional mechanisms of biological systems.
Dimitrios Kontziampasis +9 more
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Towards light-coupled sample preparation for time-resolved cryoEM studies [PDF]
IUCrJThe dynamic nature of protein and macromolecular complexes means that the capture of multiple sequential states along a reaction pathway can provide much greater insight into function than that obtained from a single static structure. We present a set of
Kyprianos Hadjidemetriou +9 more
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Time-Resolved Studies of Ytterbium Distribution at Interfacial Surfaces of Ferritin-like Dps Protein Demonstrate Metal Uptake and Storage Pathways [PDF]
Biomedicines, 2021 Cage-shaped protein (CSP) complexes are frequently used in bionanotechnology, and they have a variety of different architectures and sizes. The smallest cage-shaped protein, Dps (DNA binding protein from starved cells), can naturally form iron oxide ...
Kornelius Zeth +2 more
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Time-resolved studies of light propagation in paper [PDF]
Applied Optics, 1995 A method for time-resolved recording of light scattering in thin, highly scattering media is described. Subpicosecond pulses from a high-power Ti:sapphire laser are used, and single-shot recordings of the scattered light are made with a fast streak camera.
Carlsson, J +5 more
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Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles [PDF]
IUCrJRecent progress in gas dynamic virtual nozzle (GDVN) technologies in combination with high-brilliance synchrotron and X-ray free-electron lasers (XFELs) has allowed the visualization of protein dynamics in crystallo by mixing macromolecular protein ...
Jacob A. Summers +10 more
doaj +2 more sources
Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography [PDF]
IUCrJWe describe the design and implementation of a drop-on-fixed-target method for time-resolved serial crystallography at both synchrotron and XFEL facilities.
Jos J. A. G. Kamps +20 more
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