Results 91 to 100 of about 725,998 (282)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Design and Evaluation of a Mobile Photo Gallery in TIP

open access: yes, 2007
As a part of the Tourist Information Provider (TIP) system, this project focuses on creating a photo gallery service in the TIP system, which allows users to share, browse and categorize their photos.
Wang, Yi
core  

About the effect of plastic dissipation in heat at the crack tip on the stress intensity factor under cyclic loading [PDF]

open access: yes, 2013
Please cite this article as: Ranc, N., Palin-Luc, T., Paris, P.C., Saintier, N., About the effect of plastic dissipation in heat at the crack tip on the stress intensity factor under cyclic loading, International Journal of Fatigue (2013).Because of the ...
PARIS, Paul C.   +7 more
core   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

open access: yesFEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura   +19 more
wiley   +1 more source

Effect of different electrode tip angles with tilted torch in stationary gas tungsten arc welding : A 3D simulation

open access: yes, 2013
In this study, the effect of different tip angles (30°, 60°, 90° and 120°) on the arc and weld pool behaviour is analysed in 2mm and 5mm arc lengths with tilted (70°) torch.
Parvez, Shahid   +2 more
core   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

open access: yesFEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo   +6 more
wiley   +1 more source

Simulation of the response of the inner hair cell stereocilia bundle to an acoustical stimulus.

open access: yesPLoS ONE, 2011
Mammalian hearing relies on a cochlear hydrodynamic sensor embodied in the inner hair cell stereocilia bundle. It is presumed that acoustical stimuli induce a fluid shear-driven motion between the tectorial membrane and the reticular lamina to deflect ...
Sonya T Smith, Richard S Chadwick
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Nonlinear Vibrations of Low Pressure Turbine Bladed Disks: Tests and Simulations

open access: yesApplied Sciences
One of the most effective methods to limit the mechanical vibrations of bladed disks is the use of friction damping at mechanical joint interfaces. Unfortunately, dedicated tests to assess the impact of mistuning and the effectiveness of friction dampers
Umidjon Usmanov   +5 more
doaj   +1 more source

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