Results 81 to 90 of about 5,608 (193)
Postsynaptic Complexin Mediates Constitutive Exocytosis of Nicotinic Acetylcholine Receptor
Beyond its established presynaptic role, complexin is identified as a postsynaptic regulator of neurotransmitter receptor trafficking. By promoting constitutive and activity‐dependent exocytosis of nicotinic acetylcholine receptors, complexin controls spontaneous synaptic signaling at the C.
Ya Wang +4 more
wiley +1 more source
Analysis of the interaction between TIR1/AFBs and Aux/IAAs in Arabidopsis thaliana [PDF]
The plant hormone auxin is vital for all aspects of plant development and growth. Auxin mediates the interaction between the F-box proteins TIR1/AFB with the Aux/IAA transcription repressors and further induces the degradation of Aux/IAAs through the ...
Yu, Hong
core
TIR1 oligomerization is essential for auxin signaling.
(a) Semi in vitro pull-down assays using GST-IAA3 and Nicotiana cell extracts expressing wild-type and mutant HA:TIR1 proteins. Values represent fold-change compared to TIR1:HA samples (all values were corrected for equal loading among samples).
Britney L. Moss (3115362) +6 more
core +1 more source
Summary: Indole-3-propionic acid (IPA) is known to be a microbe-derived compound with a similar structure to the phytohormone auxin (indole-3-acetic acid, IAA). Previous studies reported that IPA exhibited auxin-like bioactivities in plants. However, the
Yue Sun +7 more
doaj +1 more source
A set of spatially-clustered amino acids are critical for TIR1 oligomerization.
(a, c, e, g, i and k) BiFC-based assessment of TIR1 oligomerization using wild-type and G142A, F143A, I151A, V117A and G147A mutants, respectively, following transient expression in Nicotiana leaves.
Britney L. Moss (3115362) +6 more
core +1 more source
The auxin-inducible degron (AID) system is a powerful tool to induce targeted degradation of proteins in eukaryotic model organisms. The efficiency of the existing Schizosaccharomyces pombe AID system is limited due to the fusion of the F-box protein ...
John Spencer (4461472) +3 more
core +2 more sources
Auxin-inducible protein depletion system in fission yeast
Background Inducible inactivation of a protein is a powerful approach for analysis of its function within cells. Fission yeast is a useful model for studying the fundamental mechanisms such as chromosome maintenance and cell cycle.
Kakimoto Tatsuo +6 more
doaj +1 more source
Adaptation of the AID system for stem cell and transgenic mouse research
The auxin-inducible degron (AID) system is becoming a widely used method for rapid and reversible degradation of target proteins. This system has been successfully used to study gene and protein functions in eukaryotic cells and common model organisms ...
Marina V. Pryzhkova +2 more
doaj +1 more source
ABSTRACT Hydrogen gas (H2) effectively alleviates abiotic stress in horticultural plants. Protein S‐nitrosylation, a key post‐translational modification, serves as a critical mechanism for nitric oxide (NO) to exert its biological function under adverse conditions.
Hua Fang +12 more
wiley +1 more source
Time‐ and space‐resolved transcriptional regulation in Arabidopsis thaliana
Transcript abundance patterns in time and space of kinases, E3 ligases and transcription factors in Arabidopsis thaliana demonstrate ample room for dynamic and specific regulation of metabolism and stress responses. Abstract General metabolism and responses to internal or external signals are tightly regulated in plants.
S. Zenker, K. Schiller, A. Bräutigam
wiley +1 more source

