Guidelines for the use of cell lines in biomedical research [PDF]
, 2014 Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture.
Capes-Davis, A. +11 more
core +2 more sources
Xenofree generation of limbal stem cells for ocular surface advanced cell therapy
Stem Cell Research & Therapy, 2019 Background Limbal stem cells (LSC) sustain the corneal integrity and homeostasis. LSC deficiency (LSCD) leads to loss of corneal transparency and blindness. A clinical approach to treat unilateral LSCD comprises autologous cultured limbal epithelial stem
Nuria Nieto-Nicolau +5 more
doaj +1 more source
The novel MAPT mutation K298E:mechanisms of mutant tau toxicity, brain pathology and tau expression in induced fibroblast-derived neurons [PDF]
, 2013 Frontotemporal lobar degeneration (FTLD) consists of a group of neurodegenerative diseases characterized by behavioural and executive impairment, language disorders and motor dysfunction.
Calo, Laura +14 more
core +4 more sources
Tibial Periosteum For The Surgical Perforation [PDF]
, 2016 Purpose: Report a successful case of scleral perforation repair, refractive to treatment with bank-scleral graft, using pretibial periosteum graft patch.
Mateo A +5 more
core +1 more source
Comparing computer-generated and pathologist-generated tumour segmentations for immunohistochemical scoring of breast tissue microarrays [PDF]
, 2015 BACKGROUND: Tissue microarrays (TMAs) have become a valuable resource for biomarker expression in translational research. Immunohistochemical (IHC) assessment of TMAs is the principal method for analysing large numbers of patient samples, but manual IHC ...
A Madabhushi +20 more
core +3 more sources
Exaggerated CpH methylation in the autism-affected brain. [PDF]
, 2017 BackgroundThe etiology of autism, a complex, heritable, neurodevelopmental disorder, remains largely unexplained. Given the unexplained risk and recent evidence supporting a role for epigenetic mechanisms in the development of autism, we explored the ...
Arking, Dan E +4 more
core +1 more source
Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion. [PDF]
, 2020 The advent of cell culture-based methods for the establishment and expansion of human corneal endothelial cells (CEnC) has provided a source of transplantable corneal endothelium, with a significant potential to challenge the one donor-one recipient ...
Aldave, Anthony J +14 more
core
Clinical Case Reports, 2020 Optimization of Hematology Patient's treatment: It is possible to obtain a 100% CD34+ recovery after CD34+ selection using the CliniMACS Prodigy.
Oscar M. Pello +8 more
doaj +1 more source
Mumio (Shilajit) as a potential chemotherapeutic for the urinary bladder cancer treatment
Scientific Reports, 2021 Mumio (Shilajit) is a traditional medicinal drug known and used for hundreds of years. Bladder cancer is one of the most common cancer types and better treatments are needed.
T. Kloskowski +7 more
doaj +1 more source
Peptide‐based ligand antagonists block a Vibrio cholerae adhesin
FEBS Letters, EarlyView.The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang +9 more
wiley +1 more source