Results 251 to 260 of about 405,445 (304)

A technique for the freeze-fracture of tissue culture

Journal of Cell Science, 1978
ABSTRACT Cultures of rat cerebellum grown on glass coverslips coated first with collodion and then collagen are fixed in glutaraldehyde to which ruthenium red has been added. After fixation, the collodion comes off the glass coverslips and the 3 layers: collodion-collagen-culture are handled as one piece. Following immersion in glycerol,
L, Prescott, M W, Brightman
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Mammalian Cell Tissue Culture Techniques

Current Protocols in Molecular Biology, 2016
AbstractCultured mammalian cells are used extensively in molecular biology studies. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This appendix describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with
Katy, Phelan, Kristin M, May
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Mutagenesis Techniques in Plant Tissue Cultures

2003
Mutant plant cell cultures can be useful in the study of the physiology and genetics of plants, as well for the improvement of crops. For recent reviews of mutant cell selection from tissue cultures, the reader is referred to Duncan and Widholm (1), Bright et al. (2), Flick (3), and Chapters 39 - 42 , in this vol. Often these mutants have been obtained
J M, Nelshoppen, J M, Widholm
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Electrophysiological Techniques in Dissociated Tissue Culture

1989
The application of electrophysiological techniques to tissue culture is still evolving. We have attempted in this chapter to give a practical summary of intracellular recording techniques used in our laboratory, as well as give some examples of new experimental strategies and electrophysiological methods that should provide further information on a ...
G L, Westbrook, P G, Nelson
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Overview of Cell and Tissue Culture Techniques

Current Protocols in Pharmacology, 1999
AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
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Techniques for Mammalian Cell Tissue Culture

Current Protocols in Human Genetics, 1994
AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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Tissue Culture Techniques

1982
Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels.
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A novel technique to overcome browning in tissue culture

Plant Cell Reports, 1991
Experiments conducted using Dioscorea alata L. revealed that an exudate from the cut end of the explants was responsible for browning of the culture medium. Browning did not affect growth of roots and shoots when explants were cultured in a large volume of medium, but in a small volume it was lethal.
S R, Bhat, K P, Chandel
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Tissue Culture Techniques: Introduction

2021
Cell culture techniques were developed as a way to study animal cells in vitro. The behavior of single cells, organ culture and monolayer cells can be studied without the in vivo influences arising from physiological and environmental variations among experimental animals.
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Clonal propagation of Callistemon by tissue culture techniques

Plant Cell Reports, 1982
Callus development in Callistemon viminalis was readily achieved when axillary buds derived from nodal tissue were placed in a medium containing macro- and micro-nutrients, sucrose (0.06 M), inositol (300 μM), nicotinic acid (20 μM), pyridoxine hydrochloride (3 μM), thiamine hydrochloride (2 μM), riboflavin (10 μM), cytokinins (5 μM) and auxins (0.1 μM)
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