Results 61 to 70 of about 405,445 (304)

Development of primary cell cultures using hemocytes and phagocytic tissue cells of Locusta migratoria: an application for locust immunity studies

, 2016
Insect cell cultures played central roles in unraveling many insect physiological and immunological processes. Regardless, despite imminent needs, insect cell lines were developed primarily from Dipteran and Lepidopteran orders, leaving many important ...
Huybrechts, Roger, Duressa, T.
core   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Unfolding phenolics biosynthetic plasticity of Artemisia alba Turra through plant tissue culture techniques

Artemisia alba Turra is an essential oil bearing plant, with Euro-Mediterranean and Southeastern European distribution. One of the distinctive characteristics of its essential oil is the variability of its terpenoid profile due to environmental, genetic ...
Todorova, Milka, Danova, Kalina, Trendafilova, Antoaneta, Wolfram, Evelyn, Peter, Samuel, Ivanova, Viktoria   +5 more
core   +1 more source

Simulation and Statistical Techniques to Explore Lymphoid Tissue Organogenesis [PDF]

, 2012
Secondary lymphoid organs have a key role in the initiation of adaptive immune responses to infection. Organogenesis occurs in foetal development, and the use of genetic tools, imaging technologies, and ex vivo culture systems has provided significant ...
Alden, Kieran
core  

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus Repair

Orthopaedic Surgery, 2020
Objective To compare the ability of three culture strategies of static culture, intermittent centrifugal culture and dynamic bioreactor culture in promoting the infiltration of bone marrow mesenchymal stem cells (BMSCs) throughout electrospun nanoporous ...
Shuang Wang, Yun‐Fei He, Jun Ma, Lei Yu, Jian‐Kun Wen, Xiao‐Jian Ye   +5 more
doaj   +1 more source

Development of tetraploids in tissue culture: modern techniques and biotechnological innovations

Polyploidy, characterized by chromosome multiplication, plays a pivotal role in plant evolution and agricultural innovation. This review explores advanced methodologies for tetraploid development in tissue culture, highlighting chemical inducers such as ...
Mohamed, Heba I., Baloch, Faheem Shehzad, Soomro, Salma Naimatullha, Liaqat, Waqas, Nadeem, Muhammad Azhar, Altaf, Muhammad Tanveer, Soomro, Sabeen Rehman   +6 more
core   +1 more source

Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapy

Molecular Oncology, EarlyView.
We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, Katrin Kleinmanns   +10 more
wiley   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Sensitivities, specificities, and predictive values of microbiological culture techniques for the diagnosis of prosthetic joint infection [PDF]

, 2014
Background. Identifying the microorganism in a prosthetic joint infection is the key to appropriately targeting antimicrobial treatment. Despite the availability of various techniques, no single test is considered the definitive gold standard. Aim.
Foguet, P, Jordan, Robert W., Smith, NA, Sprowson, AP, Saithna, A, Saithna, Adnan, Foguet, Pedro, Jordan, RW, Smith, Nicholas A., Sprowson, Andrew P.   +9 more
core   +1 more source