Formalin-free tissue embedding is less hazardous and results in better DNA quality. [PDF]
PLoS ONEDisease diagnosis, prognosis, and therapy choice progressively rely on good-quality Deoxyribonucleic acid (DNA) for molecular analysis. As tissue processing is routinely performed worldwide with ancient techniques using toxic and DNA-damaging ...
Maarten Niemantsverdriet +3 more
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CELLama: Foundation Model for Single Cell and Spatial Transcriptomics by Cell Embedding Leveraging Language Model Abilities [PDF]
Advanced ScienceLarge‐scale single‐cell RNA sequencing (scRNA‐seq) and spatial transcriptomics (ST) have transformed biomedical research into a data‐driven field, enabling the creation of comprehensive atlases.
Jeongbin Park +7 more
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Cyclooxygenase-2 and von Willebrand factor—an immunohistochemical study of the equine foot with and without laminitis, post-mortem perfused with paraffin oil [PDF]
Frontiers in Veterinary ScienceObjectiveEquine laminitis is a complex and potentially fatal disease characterized by severe vascular and inflammatory alterations within the equine foot.
Bianca A. Underberg +4 more
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Fibrin scaffolds for angiogenesis in soft tissue models: a systematic review [PDF]
Bioactive MaterialsBackground: Fibrin is a biocompatible, angiogenic biomaterial widely used in soft tissue engineering, with outcomes influenced by scaffold formulation and design.
Carla Verónica Fuenteslópez +3 more
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A comprehensive benchmark of single-cell Hi-C embedding tools [PDF]
Nature CommunicationsEmbedding is the key step in single-cell Hi-C (scHi-C) analysis which relies on capturing biological meaningful heterogeneity at various levels of genome architecture.
Dylan Plummer +5 more
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Sampling and ranking spatial transcriptomics data embeddings to identify tissue architecture
Frontiers in Genetics, 2022 Spatial transcriptomics is an emerging technology widely applied to the analyses of tissue architecture and corresponding biological functions. Substantial computational methods have been developed for analyzing spatial transcriptomics data.
Yu Lin +10 more
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Scientific Reports, 2023 The combination of MALDI mass spectrometry imaging, laser-capture microdissection, and quantitative proteomics allows the identification and characterization of molecularly distinct tissue compartments.
Francesco Greco +3 more
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Review of Post-embedding Immunogold Methods for the Study of Neuronal Structures
Frontiers in Neuroanatomy, 2021 The post-embedding immunogold (PI) technique for immunolabeling of neuronal tissues utilizing standard thin-section transmission electron microscopy (TEM) continues to be a prime method for understanding the functional localization of key proteins in ...
Ronald S. Petralia, Ya-Xian Wang
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Methods and Protocols, 2023 A histological examination is an important tool in embryology, developmental biology, and correlated areas. Despite the amount of information available about tissue embedding and different media, there is a lack of information regarding best practices ...
Melyssa Kmecick +4 more
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Advancements in 3D spheroid imaging: Optimised cryosectioning and immunostaining techniques
MethodsX, 2023 This article presents a modified protocol for embedding and sectioning spheroids and organoids, which are increasingly used in research due to their ability to emulate living tissue.
Claire Charlet-Faure +2 more
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