Results 141 to 150 of about 242,429 (195)

Multiparameter Immunofluorescence on Paraffin-Embedded Tissue Sections

Applied Immunohistochemistry & Molecular Morphology, 2006
Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations.
Frauke, Bataille, Sabine, Troppmann, Frank, Klebl, Gerhard, Rogler, Benjamin, Stoelcker, Ferdinand, Hofstadter, Anja-Katrin, Bosserhoff, Petra, Rümmele   +7 more
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DNA Extraction from Paraffin-Embedded Tissues

2003
In routine histopathology, most tissues are fixed in formalin and embedded in paraffin for long-term preservation. DNA can be extracted from these tissues for subsequent molecular analysis by amplification methods. We describe herein a protocol for DNA preparation from paraffin-embedded tissues based on published procedures (1-3).
H, Fan, M L, Gulley
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Lowicryl embedding of gastrointestinal tissues

Journal of Electron Microscopy Technique, 1990
AbstractIn the present study, we modified the technique described by Altman et al. (J. Histochem. Cytochem., 32:1217–1223, 1984) for rapid embedding of tissues in Lowicryl K4M. To attain good sections of the small intestine that contained villi, crypts, submucosa, and external muscle layers, we cut 100 μm slices of the full thickness of the wall with a
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Tissue Processing: V. Embedding

1991
Embedding of tissue enables it to withstand sectioning. One simple approach is to freeze the material and section it in a cryostat (Sects.11.2.2 and 11.3.1). The formation of ice has an immobilizing effect and also creates an embedding medium. Embedding in its more traditional sense implies the use of gelatin, paraffin, or plastic materials.
Lyon, Hans, van Deurs, Bo, Høyer, Poul Erik, Prentø, Poul, Møller, Morten   +4 more
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Real-time immunohistochemistry analysis of embedded tissue

Applied Radiation and Isotopes, 2010
We present a novel analysis of membrane-protein expression in tissue sections based on semi-automatic real-time measurement using LigandTracer(®) technology. A commercial antiHER2 antibody developed for immunohistochemistry used in this setup was revealed to have sub-optimal interaction with tissue when analyzed as recommended for immunohistochemistry.
Lars, Gedda, Hanna, Björkelund, Karl, Andersson   +2 more
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Ideal cooling process for paraffin-embedded tissues

Pathology & Oncology Research, 2004
Back in the seventies everybody was convinced that it was no longer necessary to cool paraffinembedded tissues, because of the new advances in the production of paraffin. The reason for this assumption was the addition of plastic polymers and dimethyl sulfoxide. The quality of tissue sectioning improved because of these additives.
Ferenc, Brittig, Philip, Weinkauf, Hannelore, Kärchner   +2 more
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Enzyme Histochemistry on Paraffin Embedded Tissue Sections

Stain Technology, 1981
To obtain diagnostic enzyme reactions in paraffin embedded tissue sections, we compared four fixatives (buffered formol sucrose, Baker's formol calcium, periodate lysin paraformaldehyde, and buffered formalin acetone) and subsequent acetone dehydration with or without graded concentrations of Triton X-100.
T, Fujimori, T, Mochino, M, Miura, I, Katayama   +3 more
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Fixation, Embedding, and Sectioning of Plant Tissues

Cold Spring Harbor Protocols, 2008
INTRODUCTIONThis protocol describes the preparation of plant tissues for sectioning and subsequent use in immunohistochemistry or in situ hybridization. Tissues of interest are fixed and embedded in Paraplast before sectioning.
Detlef, Weigel, Jane, Glazebrook
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Interphase Cytogenetics in Paraffin-Embedded Ovarian Tissue

2003
Interphase cytogenetics using formalin-fixed/paraffin-embedded tissue is now a well-established technique, which renders it possible to obtain "cytogenetic information" from interphase nuclei of solid tumors (1,2, for ovarian cancer, e.g., 3-8). It is the only tool to investigate specific numerical chromosomal aberrations (3-7), amplification.
S, Neubauer, T, Liehr
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Embedding Mouse Embryos and Tissues in Wax

Cold Spring Harbor Protocols, 2007
INTRODUCTIONThis protocol describes how to embed mouse tissues and embryos (large and small) in wax. The specimens must be dehydrated prior to embedding. Embedded samples are subsequently sectioned and used for in situ hybridization and immunohistochemistry experiments.
Andras, Nagy, Marina, Gertsenstein, Kristina, Vintersten, Richard, Behringer   +3 more
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