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Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections
2018Planarians are flatworms with almost unlimited regenerative abilities, which make them an excellent model for stem cell-based regeneration. To study the process of regeneration at the cellular level, immunohistochemical staining methods are an important tool, and the availability of such protocols is one of the prerequisites for mechanistic experiments
Teresa, Adell +5 more
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Polyethylene glycol embedded tissue sections for immunoelectronmicroscopy
Histochemistry, 1981Several methods for tissue embedding in polyethylene glycol (PEG) were compared with regard to their applicability for pre-embedding immunoelectronmicroscopy. Existing embedding procedures gave unsatisfactory results and therefore a modified procedure was developed.
F T, Bosman, P M, Go
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Immunohistochemistry On Glycol Methacrylate Embedded Tissues: Possibilities and Limitations
Journal of Histotechnology, 1988Many variables contribute to the successful immunostaining of water-miscible glycol methacrylate embedded sections. These variables (fixations, dehydration, infiltration, temperature, antigenic sites, molecule size, and resin composition) and their effects on antigenicity are discussed. An extensive review of the literature is included.
Gerrits, Peter O., Van Goor, Harry
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Generating embedded urban tissue
2005UCL (University College London) London - http://128.40.59.163/cupum/searchPapers/detail.asp?pID ...
DIAPPI, LIDIA, DERIX C., COATES P.
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Fluorescent Microscopy of Glycol Methacrylate-embedded Tissue
American Journal of Clinical Pathology, 1980Formalin fixed, glycol methacrylate-embedded tissue samples can easily be used for immunofluorescent studies by direct and indirect fluorescein isothiocyanate staining after treatment with protease V to unmask antigenicity. It is, therefore, possible to use serial 2-micron glycol methacrylate-embedded sections for immunologic studies, as well as ...
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Embedding of Tissue in Histopathology
2018This chapter discusses the embedding of the tissue in the medium to make a block for tissue sectioning and archival preservation of the representative tissue. The choice of the embedding medium of the tissue depends on type of tissue, type of microtomy and also type of microscope to examine the tissue.
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A rapid connective tissue stain for glycol methacrylate embedded tissue.
Acta morphologica Neerlando-Scandinavica, 1987No reliable connective tissue stains for GMA sections were available until recently. However, the use of toluidine blue in combination with basic fuchsin appeared to be a rapid and reliable connective tissue stain for glycol methacrylate (GMA) embedded tissue.
Blaauw, E. H. +2 more
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A Silver Method for Counterstaining Plastic Embedded Tissue
Stain Technology, 1983This report presents a method which can be used for counterstaining semithin sections of plastic embedded tissue. The sections are treated with a solution of silver lactate, followed by physical development. During the silver lactate treatment, silver ions are bound by various tissue components as metallic silver or silver sulfide.
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Embedding of Tissues in Carbowax
American Journal of Clinical Pathology, 1959R M, JONES, W A, THOMAS, R M, O'NEAL
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Integrative oncology: Addressing the global challenges of cancer prevention and treatment
Ca-A Cancer Journal for Clinicians, 2022Jun J Mao,, Msce +2 more
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