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Using spatial proteomics to enhance cell type assignments in histology images. [PDF]
Dayao MT +3 more
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SURGICAL OUTCOMES OF LAMELLAR MACULAR HOLE EYES WITH OR WITHOUT PRESERVATION OF LAMELLAR HOLE-ASSOCIATED EPIRETINAL PROLIFERATION TECHNIQUE: A Meta-analysis. [PDF]
Yu Y, Wang YY, Tian M, Yu XH.
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Critical point drying of brain tissue for X-ray phase contrast imaging
Khan S +16 more
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Softening Paraffin-Embedded Plant Tissues
Stain Technology, 1953Soaking paraffin-embedded plant specimens 2–3 days at 37°C. in a mixture of glycerol, 10 ml., Dreft, 1 g., and water 90 ml. is an effective means of softening them prior to sectioning. One side of the paraffin block must be pared away to expose the tissue before immersion in the softening solution.
S M, ALCORN, P A, ARK
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Decalcifying Tissues for Paraffin Embedding
Cold Spring Harbor Protocols, 2008INTRODUCTIONParaffin sections of bone usually require a decalcification step after fixation before sectioning. This protocol describes a method for decalcifying fixed tissue.
Fischer, Andrew H. +3 more
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Paraffin Embedding Tissue Samples for Sectioning
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes a method for embedding tissues in paraffin blocks for sectioning. Paraffin sections require extensive fixation and processing steps, but provide superior morphology compared with other sectioning methods.
Fischer, Andrew H. +3 more
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Cutting Sections of Paraffin-Embedded Tissues
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes the sectioning of tissues embedded in paraffin blocks. Paraffin sections require extensive fixation and processing steps but provide superior morphology compared with other sectioning methods. Sectioning paraffin blocks requires experience and should be learned from an experienced researcher, if possible.
Fischer, Andrew H. +3 more
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Multiparameter Immunofluorescence on Paraffin-Embedded Tissue Sections
Applied Immunohistochemistry & Molecular Morphology, 2006Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations.
Frauke, Bataille +7 more
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DNA Extraction from Paraffin-Embedded Tissues
2003In routine histopathology, most tissues are fixed in formalin and embedded in paraffin for long-term preservation. DNA can be extracted from these tissues for subsequent molecular analysis by amplification methods. We describe herein a protocol for DNA preparation from paraffin-embedded tissues based on published procedures (1-3).
H, Fan, M L, Gulley
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Lowicryl embedding of gastrointestinal tissues
Journal of Electron Microscopy Technique, 1990AbstractIn the present study, we modified the technique described by Altman et al. (J. Histochem. Cytochem., 32:1217–1223, 1984) for rapid embedding of tissues in Lowicryl K4M. To attain good sections of the small intestine that contained villi, crypts, submucosa, and external muscle layers, we cut 100 μm slices of the full thickness of the wall with a
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