Results 241 to 250 of about 242,093 (272)

Tissue Processing: V. Embedding

1991
Embedding of tissue enables it to withstand sectioning. One simple approach is to freeze the material and section it in a cryostat (Sects.11.2.2 and 11.3.1). The formation of ice has an immobilizing effect and also creates an embedding medium. Embedding in its more traditional sense implies the use of gelatin, paraffin, or plastic materials.
Lyon, Hans, van Deurs, Bo, Høyer, Poul Erik, Prentø, Poul, Møller, Morten   +4 more
openaire   +1 more source

Real-time immunohistochemistry analysis of embedded tissue

Applied Radiation and Isotopes, 2010
We present a novel analysis of membrane-protein expression in tissue sections based on semi-automatic real-time measurement using LigandTracer(®) technology. A commercial antiHER2 antibody developed for immunohistochemistry used in this setup was revealed to have sub-optimal interaction with tissue when analyzed as recommended for immunohistochemistry.
Lars, Gedda, Hanna, Björkelund, Karl, Andersson   +2 more
openaire   +2 more sources

Ideal cooling process for paraffin-embedded tissues

Pathology & Oncology Research, 2004
Back in the seventies everybody was convinced that it was no longer necessary to cool paraffinembedded tissues, because of the new advances in the production of paraffin. The reason for this assumption was the addition of plastic polymers and dimethyl sulfoxide. The quality of tissue sectioning improved because of these additives.
Ferenc, Brittig, Philip, Weinkauf, Hannelore, Kärchner   +2 more
openaire   +2 more sources

Enzyme Histochemistry on Paraffin Embedded Tissue Sections

Stain Technology, 1981
To obtain diagnostic enzyme reactions in paraffin embedded tissue sections, we compared four fixatives (buffered formol sucrose, Baker's formol calcium, periodate lysin paraformaldehyde, and buffered formalin acetone) and subsequent acetone dehydration with or without graded concentrations of Triton X-100.
T, Fujimori, T, Mochino, M, Miura, I, Katayama   +3 more
openaire   +2 more sources

Fixation, Embedding, and Sectioning of Plant Tissues

Cold Spring Harbor Protocols, 2008
INTRODUCTIONThis protocol describes the preparation of plant tissues for sectioning and subsequent use in immunohistochemistry or in situ hybridization. Tissues of interest are fixed and embedded in Paraplast before sectioning.
Detlef, Weigel, Jane, Glazebrook
openaire   +2 more sources

Interphase Cytogenetics in Paraffin-Embedded Ovarian Tissue

2003
Interphase cytogenetics using formalin-fixed/paraffin-embedded tissue is now a well-established technique, which renders it possible to obtain "cytogenetic information" from interphase nuclei of solid tumors (1,2, for ovarian cancer, e.g., 3-8). It is the only tool to investigate specific numerical chromosomal aberrations (3-7), amplification.
S, Neubauer, T, Liehr
openaire   +2 more sources

Embedding Mouse Embryos and Tissues in Wax

Cold Spring Harbor Protocols, 2007
INTRODUCTIONThis protocol describes how to embed mouse tissues and embryos (large and small) in wax. The specimens must be dehydrated prior to embedding. Embedded samples are subsequently sectioned and used for in situ hybridization and immunohistochemistry experiments.
Andras, Nagy, Marina, Gertsenstein, Kristina, Vintersten, Richard, Behringer   +3 more
openaire   +2 more sources

Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections

2018
Planarians are flatworms with almost unlimited regenerative abilities, which make them an excellent model for stem cell-based regeneration. To study the process of regeneration at the cellular level, immunohistochemical staining methods are an important tool, and the availability of such protocols is one of the prerequisites for mechanistic experiments
Teresa, Adell, Sara, Barberán, Miquel, Sureda-Gómez, María, Almuedo-Castillo, Nidia, de Sousa, Francesc, Cebrià   +5 more
openaire   +2 more sources

Polyethylene glycol embedded tissue sections for immunoelectronmicroscopy

Histochemistry, 1981
Several methods for tissue embedding in polyethylene glycol (PEG) were compared with regard to their applicability for pre-embedding immunoelectronmicroscopy. Existing embedding procedures gave unsatisfactory results and therefore a modified procedure was developed.
F T, Bosman, P M, Go
openaire   +2 more sources

Immunohistochemistry On Glycol Methacrylate Embedded Tissues: Possibilities and Limitations

Journal of Histotechnology, 1988
Many variables contribute to the successful immunostaining of water-miscible glycol methacrylate embedded sections. These variables (fixations, dehydration, infiltration, temperature, antigenic sites, molecule size, and resin composition) and their effects on antigenicity are discussed. An extensive review of the literature is included.
Gerrits, Peter O., Van Goor, Harry
openaire   +2 more sources