Results 31 to 40 of about 242,093 (272)
Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: Novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering [PDF]
Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles.
Athanasiadis, A. +8 more
core +1 more source
Simultaneous multi-material embedded printing for 3D heterogeneous structures
In order to mimic the natural heterogeneity of native tissue and provide a better microenvironment for cell culturing, multi-material bioprinting has become a common solution to construct tissue models in vitro .
Ziqi Gao +6 more
doaj +1 more source
Quantitative evaluation of embedding resins for volume electron microscopy
Optimal epoxy resin embedding is crucial for obtaining consistent serial sections from large tissue samples, especially for block faces spanning >1 mm2.
Lennart Tegethoff, Kevin L. Briggman
doaj +1 more source
Serial Block-face Scanning Electron Microscopy (SBF-SEM or 3D-EM) is a powerful tool to study biological structure in ultrastructural level. Quantification of cellular ultrastructure is useful to providing biological information. This technique requires
Haiyan Lu +2 more
doaj +1 more source
Mucosubstances in the porcine gastrointestinal tract: Fixation, staining and quantification [PDF]
Mucins are of great interest in intestinal research and histochemical methods are often employed to identify them. Since it is in the nature of mucins that they are “hard to hold onto” once they come into contact with water, a frequently used medium in ...
Drewes, Barbara +3 more
core +1 more source
Histological examination of targets in regions of interest in histological sections is one of the most frequently used tools in biomedical research. However, it is a technical challenge to secure a multitarget section for inspection of the structure’s ...
Zhongmin Li +4 more
doaj +1 more source
Hormone immunolabeling in resin-embedded Arabidopsis tissues
Here, we present a protocol for immunolabeling of molecules in Arabidopsis tissues. We describe steps for tissue fixation and embedding in resin of microtome-derived sections, immunolabeling using fluorescent and non-fluorescent secondary antibodies, and visualization of cytokinin and auxin molecules.
Herrera-Ubaldo, Humberto +4 more
openaire +3 more sources
A novel tissue microarray instrumentation:The HT-1 tissue microarrayer
Background: Tissue microarray (TMA) is a novel and useful tool to efficiently analyze gene expression in histological tissues. Aim: Cost-efficient and easy to use automated tissue arrayers will provide a better instrumentation to generate TMAs.
Jun Yang +4 more
doaj +1 more source
Paraffin-embedding for large volume bio-tissue [PDF]
AbstractAcquiring ultrahigh-resolution three-dimensional images of large-volume tissues non-human primate tissues was an enormous challenge. Given the preservation of structure and excellent sectioning property, formalin-fixed paraffin-embedding method had an enormous potential for three-dimensional reconstruction of fine structures, based on the very ...
Zhanmu, Ouyang +3 more
openaire +2 more sources
Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
Intranuclear birefringent inclusions (IBI) found in various cell types in paraffin-embedded tissue sections have long been considered to be a tissue processing artifact, although an association with biological processes has been suggested.
Aiste Vitkunaite +6 more
doaj +1 more source

