Results 61 to 70 of about 242,429 (195)

PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies

Scientific Reports
Histology is the gold standard for analyzing tissue structure and cell morphology. Immunostaining on thin tissue sections enables precise visualization of antigens and proteins.
Emilie Vuille-dit-Bille, Larissa Utz, Fiona E. Müllner, Valeria J. Arteaga-Moreta, Yanyan Hou, Stefan E. Spirig, Diane Ledroit-Paic, Sarah Heub, Jonas Goldowsky, Gilles Weder, Magdalena Renner   +10 more
doaj   +1 more source

Optimized Methyl methacrylate embedding of small and large undecalcified bones.

MethodsX
Methyl methacrylate (MMA) plastic embedding has been long established as a technique for the processing and histological assessment of bones. It provides the added benefit over paraffin in that it does not require decalcification of the tissue in order ...
Jackie A. Fretz, Nancy W. Troiano
doaj   +1 more source

A practical approach for the correction of iatrogenic penile skin loss in children: Scrotal embedding technique

Urology Research and Practice, 2019
The aim of this particular study is to determine the efficacy of scrotal embedding technique in children with overly deficient penile shaft skin, which takes advantage of the rich vascular supply of the scrotal layers and provides adequate tissue ...
Orhan Ziylan, Ömer Acar, Burcu Celet Özden, Tzevat Tefik, M. İrfan Dönmez, Tayfun Oktar   +5 more
doaj   +1 more source

Non-specific binding of antibodies in immunohistochemistry: Fakes and facts [PDF]

, 2011
Protocols for blocking non-specific antibody (Ab) binding in immunohistochemistry are based on rather contradictory and outdated reports. This prompted us to prove, whether non-specific Ab binding may really lead to unwanted background staining in ...
Igor Buchwalow, Markus Tiemann, Vera Samoilova, Werner Boecker   +3 more
core   +1 more source

The biological embedding of early-life socioeconomic status and family adversity in children's genome-wide DNA methylation. [PDF]

, 2018
AimTo examine variation in child DNA methylation to assess its potential as a pathway for effects of childhood social adversity on health across the life course.Materials & methodsIn a diverse, prospective community sample of 178 kindergarten ...
Adler, Nancy E, Boyce, W Thomas, Bush, Nicole R, Edgar, Rachel D, Essex, Marilyn J, Kobor, Michael S, MacIsaac, Julia L, McEwen, Lisa M, Park, Mina   +8 more
core   +1 more source

Anatomical Priors in Convolutional Networks for Unsupervised Biomedical Segmentation

, 2019
We consider the problem of segmenting a biomedical image into anatomical regions of interest. We specifically address the frequent scenario where we have no paired training data that contains images and their manual segmentations.
Dalca, Adrian V., Guttag, John, Sabuncu, Mert R.   +2 more
core   +1 more source

Xylene versus Isopropanol for Paraffin Wax Processing of Lung Tissue

Applied Sciences
The microscopic observation of lung tissue is challenging due to its fragile nature. Xylene and isopropanol are common tissue-clearing reagents used before paraffin embedding, yet no studies have compared these two reagents in lung tissue processing. Due
Qi Wang, Runchuan Gu, Franziska Olm, Nicholas Burdon Bèchet, Sandra Lindstedt   +4 more
doaj   +1 more source

An Optimized Protocol of Laser Capture Microdissection for Tissue-Specific RNA Profiling in a Radish Tap Root

STAR Protocols, 2020
Summary: Laser capture microdissection (LCM) coupled with transcriptome profiling is a powerful technique that allows for tissue-specific gene expression analysis in a complex system.
Goh Choe, Nam V. Hoang, Ji-Young Lee
doaj   +1 more source

Immunohistochemistry of matrix markers in Technovit 9100 Newregistered-embedded undecalcified bone sections

European Cells & Materials, 2003
Trabecular bone is routinely analysed by histomorphological-histometrical and immunohistochemical techniques as means of assessing the differentiation status of bone deposition and growth. Currently few embedding resins exist for which both morphological
Yang R., Davies C. M., Archer C. W., Richards R. G.   +3 more
doaj  

Functional morphology of cephalopod gills [PDF]

, 1987
There is a wealth of literature dealing with fish gills (Review, see Hoar & Randall, 1984), yet hardly anything is known about the gills of cephalopods. This is rather surprising considering the commercial importance of the cephalopods.
Eno, Nancy Clare
core