Results 231 to 240 of about 407,768 (269)

Production of recombinant human granulocyte‐macrophage colony‐stimulating factor in thermoinducible E. coli: Tuning inclusion body architecture and protein yield via dissolved oxygen control

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Recombinant protein expression in Escherichia coli frequently results in the formation of inclusion bodies (IBs), which provide high protein accumulation but often require additional steps to recover functional protein. Although culture conditions strongly influence IB physicochemical and structural characteristics, the impact of ...
Ximena Gómez‐Gil, Norma A Valdez‐Cruz, Sara Restrepo‐Pineda, Nuria Sánchez‐Puig, Rafael Vázquez‐Duhalt, Rubén D Cadena‐Nava, Néstor O Pérez, Mauricio A Trujillo‐Roldán   +7 more
wiley   +1 more source

Setup and Analysis of Human Tonsil and Adenoid-Based Immune Organoids for Studying T and B Cell-Mediated Adaptive Immunity. [PDF]

Curr Protoc
Moll M, Eichhorn KW, Send T, Baumjohann D.   +3 more
europepmc   +1 more source

Abdominal POCUS Education for Clinicians: A Systematic Review of Teaching Methods for Point‐of‐Care Abdominal Ultrasonography

Journal of Clinical Ultrasound, Volume 53, Issue 3, Page 547-561, March/April 2025.
For patients to benefit from abdominal point‐of‐care ultrasound, the ultrasound examinator must be sufficiently trained. In this systematic review, we identified advantages and disadvantages of different training methods and approaches. We conclude that thoughtful integration of the various educational methods is crucial for stakeholders and educators.
Birkir Örn Sveinsson, Olof Kristin Thorsteinsdottir, Alexander G. P. Browne, Benjamin S. B. Rasmussen, Anders Bo Nielsen, Pia Iben Pietersen   +5 more
wiley   +1 more source

Transcriptomic profiling of an in vivo diffuse large B-cell lymphoma model reveals molecular programs underlying CNS dissemination. [PDF]

Ann Hematol
Kim SJ, Pak HK, Kim H, Lee Y, Choi HS, Choi JW, Kim DE, Hyung J, Cho H, Yoon DH, Roh J, Park CS.   +11 more
europepmc   +1 more source

Effects of Crop Rotation and Hydrogel Application on Soil Biological Properties and Wheat Biomass Production in Drylands

Journal of Plant Nutrition and Soil Science, EarlyView.
ABSTRACT Background and aim Conservation agriculture (CA) is rapidly gaining traction to promote sustainable crop production, focusing on minimum soil disturbance, permanent soil cover, and strategic crop rotation. Moreover, hydrogel is increasingly being used to improve water retention, including in combination with CA, particularly in water limited ...
Tina Taghvaei, Catriona A. Macdonald, Jerzy Szejgis, Uffe N. Nielsen   +3 more
wiley   +1 more source

Next-Generation Sequencing Refines Diagnosis and Expands Precision Medicine Opportunities in Soft Tissue Sarcomas. [PDF]

Int J Mol Sci
Tesser-Gamba F, Mendes TB, Lima FT, Abib SCV, Caran EMM, Toledo SRC.   +5 more
europepmc   +1 more source

Development and Initial Psychometric Evaluation of CIAECSA: A Comprehensive Questionnaire on Attitudes, Experiences, and Contexts of Adolescent Sexuality. [PDF]

Healthcare (Basel)
Rodríguez-López CDR, Checa-Peñalver A, Pereira-Afonso MR, Lopezosa-Villajos V, Donoso-Calero I, Arroyo-Bello E, Gómez-Cantarino S.   +6 more
europepmc   +1 more source