Results 181 to 190 of about 4,098,484 (351)

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays

FEBS Open Bio, Volume 15, Issue 5, Page 690-698, May 2025.
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques, Edit Ábrahám, Hiromi Muramatsu, Daniel Youssef Bargieri, Norbert Pardi, Zoltán Lipinszki   +5 more
wiley   +1 more source

From ESP to CLIL using the Schema Theory

Revista de Lenguas para Fines Específicos, 2013
This paper explains the use of the Schema Theory in planning and preparing a Physiology lesson in a CLIL tertiary context in a Spanish university. The adopted combination of methods used in applying the theory is transferable to any context and subject ...
Nashwa Nashaat Sobhy, César Berzosa, Fiona M. Crean   +2 more
doaj  

Style-Based architectural reconfigurations [PDF]

, 2007
We introduce Architectural Design Rewriting (ADR), an approach to the design of reconfigurable software architectures whose key features are: (i) rule-based approach (over graphs); (ii) hierarchical design; (iii) algebraic presentation; and (iv ...
Bruni, Roberto, Lluch-Lafuente, Alberto, Montanari, Ugo, Tuosto, Emilio   +3 more
core  

Cost‐effective and simple flow cytometry quantification of receptor‐mediated autophagy using fluorescent tagging

FEBS Open Bio, Volume 15, Issue 4, Page 587-598, April 2025.
Mitophagy, the selective degradation of damaged mitochondria, is crucial for cellular health and function. This study introduces a rapid and efficient flow cytometry method using GFP‐BNIP3L/NIX to accurately quantify mitophagy. Our approach can be adapted to use any fluorescent‐tagged selective autophagy receptor, enabling direct and rapid analysis of ...
Mija Marinković, Ana Rožić, Denis Polančec, Ivana Novak   +3 more
wiley   +1 more source

Changes in electrophysiological markers of cognitive control after administration of galantamine

NeuroImage: Clinical, 2018
The healthy brain is able to maintain a stable balance between bottom-up sensory processing and top-down cognitive control. The neurotransmitter acetylcholine plays a substantial role in this.
Lauren E. Gascoyne, Karen J. Mullinger, Siân E. Robson, Jyothika Kumar, George C. O'Neill, Lena Palaniyappan, Peter G. Morris, Elizabeth B. Liddle, Matthew J. Brookes, Peter F. Liddle   +9 more
doaj  

HDAC4 regulates apoptosis in Acan‐CreERT2;HDAC4d/d mice with osteoarthritis by downregulating ATF4

FEBS Open Bio, Volume 15, Issue 5, Page 726-737, May 2025.
Using the AcanCreERT2;HDAC4fl/fl genetic mouse model, we further investigated the role of histone deacetylase 4 (HDAC4) in chondrocyte apoptosis. We found that knocking down HDAC4 may be associated with chondrocyte apoptosis by upregulating the activating transcription factor 4 (ATF4)/CAAT/enhancer binding protein homologous (CHOP) signaling pathway ...
Jingrui Huang, Yukun Xu, Yujia Li, Yiming Pang, Xueting Ding, Raorao Zhou, Dan Liang, Xianda Che, Yuanyu Zhang, Chunfang Wang, Wenjin Li, Pengcui Li   +11 more
wiley   +1 more source

Pharmacological effects of osimertinib on a chicken chorioallantoic membrane xenograft model with the EGFR exon‐19‐deleted advanced NSCLC mutation

FEBS Open Bio, Volume 15, Issue 5, Page 836-855, May 2025.
Osimertinib reduces angiogenesis and PDL1 expression in in ovo tumors, transforming them into ‘cold tumors’ with lower immune activity. Anatomopathological and transcriptomic analyses highlight its therapeutic impact on tumor biology. This study underscores osimertinib's potential to reshape the tumor microenvironment and provides insights into its ...
David Barthélémy, Arnaud Vigneron, Xavier Rousset, Jérome Guitton, Emmanuel Grolleau, Margaux Raffin, Julie Balandier, Gaëlle Lescuyer, Mathilde Bardou, Florence Geiguer, Sébastien Couraud, Claire Bardel, Jean Viallet, Nazim Benzerdjeb, Léa Payen   +14 more
wiley   +1 more source

Identification of inhibitors of the Salmonella FraB deglycase, a drug target

FEBS Open Bio, Volume 15, Issue 5, Page 773-792, May 2025.
A high‐throughput screen was used to identify inhibitors of Salmonella FraB, a drug target. Characterization of top hits (identified after an additional counter screen) revealed that some triazolidines, thiadiazolidines, and triazolothiadiazoles are mixed‐type inhibitors of FraB.
Jamison D. Law, Yuan Gao, Sravya Kovvali, Pankajavalli Thirugnanasambantham, Vicki H. Wysocki, Brian M. M. Ahmer, Venkat Gopalan   +6 more
wiley   +1 more source

OPTIMIZING LARGE COMBINATIONAL NETWORKS FOR K-LUT BASED FPGA MAPPING [PDF]

Optimizing by partitioning is a central problem in VLSI design automation, addressing circuit’s manufacturability. Circuit partitioning has multiple applications in VLSI design.
Alexandru E. ŞUŞU, Cornel POPESCU, George CULEA, Ioana FĂGĂRĂŞAN, Ion I. BUCUR   +4 more
core  

Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria

FEBS Open Bio, EarlyView.
The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao, Takato Ozaki, Hirofumi Fukuda, Yu Kanesaki, Munehiko Asayama   +4 more
wiley   +1 more source