Results 81 to 90 of about 127,022 (216)

Inference of the 3D pressure field exerted by a single cell from a thin membrane transverse deformation. [PDF]

Eur Phys J E Soft Matter
Bédel Q, Dupré L, Destainville N.
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Protocol to interrogate the role of creeping fat and mechanical tension in Crohn's disease using a surgical mouse model of intestinal fibrosis. [PDF]

STAR Protoc
Suh EJ, Pham B, Bauer-Rowe KE, Kim A, Liang NE, Park JY, Foster DS, Norton JA, Delitto D, Longaker MT, Hyun JS.   +10 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Development of a Unified Cardiovascular-Pupillary Model for Interpreting Pupil Size Variability as an Autonomic Marker. [PDF]

Biomed Eng Comput Biol
Hung K, Man GM, Chow DH.
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

4D CT angiography and computational biomechanics dataset for structural integrity assessment of abdominal aortic aneurysms. [PDF]

Data Brief
Jamshidian M, Wittek A, Sekhavat S, Alkhatib F, Ritter JC, Parizel PM, Mufty H, Maleux G, Fourneau I, Gizewski ER, Gassner E, Loizides A, Lutz M, Enzmann FK, Bernard F, Minvielle L, Fondanèche A, Polce J, Wood C, Miller K.   +19 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Network structure analysis of depression, anxiety, and stress symptoms among college students: identification of central and bridge symptoms. [PDF]

Front Psychiatry
Zhao B, Zhu H, Su Y, Chen J, Tu H.
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source