Results 41 to 50 of about 3,296,247 (272)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Multi-species trace gas sensing with dual-wavelength QCLs [PDF]

Applied Physics B, 2018
Instrumentation for environmental monitoring of gaseous pollutants and greenhouse gases tends to be complex, expensive, and energy demanding, because every compound measured relies on a specific analytical technique. This work demonstrates an alternative approach based on mid-infrared laser absorption spectroscopy with dual-wavelength quantum cascade ...
P. Morten Hundt, Béla Tuzson, Oleg Aseev, Chang Liu, Philipp Scheidegger, Herbert Looser, Filippos Kapsalidis, Mehran Shahmohammadi, Jérôme Faist, Lukas Emmenegger   +9 more
openaire   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Characterization of Defect Distribution in an Additively Manufactured AlSi10Mg as a Function of Processing Parameters and Correlations with Extreme Value Statistics

Advanced Engineering Materials, EarlyView.
Predicting extreme defects in additive manufacturing remains a key challenge limiting its structural reliability. This study proposes a statistical framework that integrates Extreme Value Theory with advanced process indicators to explore defect–process relationships and improve the estimation of critical defect sizes. The approach provides a basis for
Muhammad Muteeb Butt, Varma Kvvssn, Hossein Laieghi, Zia Uddin, Peyman Ansari, E. Kıvanç Sadak, Can Barış Toprak, Hüseyin Kızıl, Enrico Salvati   +8 more
wiley   +1 more source

Characterization of uncertainties in atmospheric trace gas inversions using hierarchical Bayesian methods [PDF]

, 2014
We present a hierarchical Bayesian method for atmospheric trace gas inversions. This method is used to estimate emissions of trace gases as well as "hyper-parameters" that characterize the probability density functions (PDFs) of the a priori emissions ...
Li, S., M. Rigby, Ganesan, Anita Lakshmi, S. Park, Fraser, P. J., S. Li, S. J. O'Doherty, P. K. Salameh, Prinn, Ronald G., L. P. Steele, Ganesan, A. L., O'Doherty, Simon, A. Zammit-Mangion, Krummel, P. B., P. B. Krummel, Muehle, J., Zammit-Mangion, A., Prinn, R. G., Weiss, R. F., Muhle, Jens, Steele, L. P., Rigby, M., Manning, A. J., Kim, K. -R., A. J. Manning, A. L. Ganesan, R. F. Weiss, Park, S., Manning, Alistair J., C. M. Harth, P. J. Fraser, Harth, C. M., Salameh, P. K., K.-R. Kim, O'Doherty, S. J., J. Mühle, R. G. Prinn, Kim, K.-R.   +37 more
core   +2 more sources

Dual-comb optomechanical spectroscopy

Nature Communications, 2023
Optical cavities are essential for enhancing the sensitivity of molecular absorption spectroscopy, which finds widespread high-sensitivity gas sensing applications.
Xinyi Ren, Jin Pan, Ming Yan, Jiteng Sheng, Cheng Yang, Qiankun Zhang, Hui Ma, Zhaoyang Wen, Kun Huang, Haibin Wu, Heping Zeng   +10 more
doaj   +1 more source

Thermomechanical Fatigue and Creep–Fatigue Interaction of Inconel 718 Additively Manufactured by Laser Beam Powder Bed Fusion

Advanced Engineering Materials, EarlyView.
Thermomechanical fatigue tests of laser beam powder bed fusion (PBF‐LB) Inconel 718 show that the additively manufactured material reaches almost the lifetimes of conventionally‐rolled material under no‐dwell conditions. Introducing dwell times at the maximum temperature markedly reduces the lifetimes due to pronounced grain boundary sliding associated
Stefan Guth, Tomáš Babinský, Jan Lars Riedel, Wen Hao Kan, Aijun Huang, Martin Heilmaier, Ivo Šulák   +6 more
wiley   +1 more source

Ultra-miniature and sensitive optical fiber-tip optomechanical resonant photoacoustic spectroscopy gas sensors

Photoacoustics
Optomechanical resonator (OMR)-based photoacoustic spectroscopy (PAS) sensors have garnered significant attention for ultra-sensitive and selective trace-gas detection.
Taige Li, Pengcheng Zhao, Peng Wang, Shangming Liu, Linhao Guo, Wei Jin, A. Ping Zhang   +6 more
doaj   +1 more source

Highly Sensitive MEMS Sensor Using Bimetallic Pd–Ag Nanoparticles as Catalyst for Acetylene Detection

Sensors, 2022
Acetylene detection plays an important role in fault diagnosis of power transformers. However, the available dissolved gas analysis (DGA) techniques have always relied on bulky instruments and are time-consuming.
Yuan Tian, Hui Qiao, Tao Yao, Shuguo Gao, Lujian Dai, Jun Zhao, Ying Chen, Pengcheng Xu   +7 more
doaj   +1 more source