Results 41 to 50 of about 72,114 (243)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Blockchain for drug traceability: Architectures and open challenges
Health Informatics Journal, 2021 Pharmaceutical supply chain (PSC) consists of multiple stakeholders including raw material suppliers, manufacturers, distributors, regulatory authorities, pharmacies, hospitals, and patients.
Mueen Uddin +4 more
doaj +1 more source
Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry
FEBS Letters, EarlyView.EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri +5 more
wiley +1 more source
Analysis of Hurricane Track Forecast Accuracy During the 2018 Season [PDF]
, 2019 Hurricane track forecasting has become more accurate in recent years due to technological advances in modeling methods. However, due to the complex nature of the relationship between oceanic and atmospheric variables and hurricane tracks, noteworthy ...
Unger, Jonathan, Unger, Jonathan D.
core +1 more source
Molecular Oncology, EarlyView.Dormant cancer cells can hide in distant organs for years, evading treatment and the immune system. This review highlights how signals from the surrounding tissue and immune environment keep these cells inactive or trigger their reawakening. Understanding these mechanisms may help develop therapies to eliminate or control dormant cells and prevent ...
Kanishka Tiwary +1 more
wiley +1 more source
Brucella suis Infection in Dog Fed Raw Meat, the Netherlands
Emerging Infectious Diseases, 2018 A Brucella suis biovar 1 infection was diagnosed in a dog without typical exposure risks, but the dog had been fed a raw meat–based diet (hare carcasses imported from Argentina).
Marloes A.M. van Dijk +8 more
doaj +1 more source
Global-scale modelling of melting and isotopic evolution of Earth's mantle: melting modules for TERRA [PDF]
Geoscientific Model Development, 2016 Many outstanding problems in solid-Earth science relate to the geodynamical explanation of geochemical observations. Currently, extensive geochemical databases of surface observations exist, but satisfying explanations of underlying mantle processes are ...
H. J. van Heck +3 more
doaj +1 more source
Wi-Fi and Bluetooth Contact Tracing Without User Intervention
IEEE Access, 2022 Previous contact tracing systems required the users to perform many manual actions, such as installing smartphone applications, joining wireless networks, or carrying custom user devices.
Brosnan Yuen +7 more
doaj +1 more source
Molecular Oncology, EarlyView.Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim +3 more
wiley +1 more source