FEBS Open Bio, EarlyView.dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source
Robotic Resistance Treadmill Training Improves Locomotor Function in Children With Cerebral Palsy: A Randomized Controlled Pilot Study [PDF]
, 2017 Objective To determine whether applying controlled resistance forces to the legs during the swing phase of gait may improve the efficacy of treadmill training as compared with applying controlled assistance forces in children with cerebral palsy (CP ...
Arora, Pooja +4 more
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
An individualised approach to monitoring and prescribing training in elite youth football players [PDF]
The concept of how training load affects performance is founded in the notion that training contributes to two specific outcomes, these are developed simultaneously by repeated bouts of training and act in conflict of each other; fitness and fatigue ...
Fitzpatrick, John +1 more
core
Enhancing suicide risk assessment through the use of visual metaphor : a thesis presented in partial fulfillment of the requirements for the degree of Master of Health Science in Psychology at Massey University, Albany Campus, New Zealand [PDF]
, 2016 Competent assessment and management of the risk of harm is a core competency that mental health professionals are expected to possess. However, despite this expectation, adequate training programs have been lacking for decades and, even when risk ...
Alyami, Mohsen Mohammed M
core
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
FEBS Open Bio, EarlyView.Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Applied SciencesBackground/Objectives: Master athletes in team sports represent a growing population of individuals who continue to engage in structured training and competition.
Dimitrios Pantazis +17 more
doaj +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Reliability and validity of a basketball-specific fatigue protocol simulating match load
Acta Gymnica, 2017 Background: Research on the amount and type of fatigue caused by match load depends on protocols based on time-motion analysis of match performance in a given sport.
Karel Hůlka, Michal Lehnert, Jan Bělka
doaj +1 more source