Results 1 to 10 of about 38,906 (120)
Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish. [PDF]
PLoS ONE, 2015 The type II clustered regularly interspaced short palindromic repeats (CRISPR) associated with Cas9 endonuclease (CRISPR/Cas9) has become a powerful genetic tool for understanding the function of a gene of interest.
Hirohito Kotani +4 more
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CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA
Scientific Reports, 2022 CRISPR-Cas12a systems are becoming an attractive genome editing tool for cell engineering due to their broader editing capabilities compared to CRISPR-Cas9 counterparts.
D. J. Jedrzejczyk +9 more
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Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity. [PDF]
PLoS ONE, 2017 Since its initial application in mammalian cells, CRISPR-Cas9 has rapidly become a preferred method for genome engineering experiments. The Cas9 nuclease is targeted to genomic DNA using guide RNAs (gRNA), either as the native dual RNA system consisting ...
Megan Basila +2 more
doaj +2 more sources
CRISPR Cpf1 proteins: structure, function and implications for genome editing
Cell & Bioscience, 2019 CRISPR and CRISPR-associated (Cas) protein, as components of microbial adaptive immune system, allows biologists to edit genomic DNA in a precise and specific way. CRISPR-Cas systems are classified into two main classes and six types.
Fatemeh Safari +4 more
doaj +2 more sources
Bio-Protocol, 2017 The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is the most widely used genome editing tool.
Satoshi Hara +2 more
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Journal of Analytical Science and TechnologyThe abnormally expressed microRNAs (miRNAs) serve as crucial indicators for disease diagnosis and are strongly associated with the progression of various diseases.
Yunxiao Li, Qiuxia Wang, Yali Wang
doaj +2 more sources
Nature Biotechnology, 2021 Gene therapy would benefit from a miniature CRISPR system that fits into the small adeno-associated virus (AAV) genome and has high cleavage activity and specificity in eukaryotic cells.
Do Yon Kim +9 more
semanticscholar +2 more sources
Comparative genomics and evolution of trans-activating RNAs in Class 2 CRISPR-Cas systems
RNA Biology, 2018 Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems.
Guilhem Faure +6 more
semanticscholar +1 more source
Structural basis for RNA-guided DNA cleavage by IscB-ωRNA and mechanistic comparison with Cas9
Science, 2022 Class 2 CRISPR effectors Cas9 and Cas12 may have evolved from nucleases in IS200/IS605 transposons. IscB is about two-fifths the size of Cas9 but shares a similar domain organization.
G. Schuler, Chunyi Hu, A. Ke
semanticscholar +1 more source
iScience, 2021 Summary: Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes bound to CRISPR RNA (crRNA) recognize specific nucleic acid targets, initiating ...
Eric A. Nalefski +10 more
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