Results 1 to 10 of about 43,663 (164)

Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish. [PDF]

PLoS ONE, 2015
The type II clustered regularly interspaced short palindromic repeats (CRISPR) associated with Cas9 endonuclease (CRISPR/Cas9) has become a powerful genetic tool for understanding the function of a gene of interest.
Hirohito Kotani, Kiyohito Taimatsu, Rie Ohga, Satoshi Ota, Atsuo Kawahara   +4 more
doaj   +2 more sources

CRISPR Cpf1 proteins: structure, function and implications for genome editing

Cell & Bioscience, 2019
CRISPR and CRISPR-associated (Cas) protein, as components of microbial adaptive immune system, allows biologists to edit genomic DNA in a precise and specific way. CRISPR-Cas systems are classified into two main classes and six types.
Fatemeh Safari, Khadijeh Zare, Manica Negahdaripour, Mazyar Barekati-Mowahed, Younes Ghasemi   +4 more
doaj   +2 more sources

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity. [PDF]

PLoS ONE, 2017
Since its initial application in mammalian cells, CRISPR-Cas9 has rapidly become a preferred method for genome engineering experiments. The Cas9 nuclease is targeted to genomic DNA using guide RNAs (gRNA), either as the native dual RNA system consisting ...
Megan Basila, Melissa L Kelley, Anja van Brabant Smith   +2 more
doaj   +2 more sources

CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA

Scientific Reports, 2022
CRISPR-Cas12a systems are becoming an attractive genome editing tool for cell engineering due to their broader editing capabilities compared to CRISPR-Cas9 counterparts.
D. J. Jedrzejczyk, L. D. Poulsen, M. Mohr, N. D. Damas, S. Schoffelen, A. Barghetti, R. Baumgartner, B. T. Weinert, T. Warnecke, R. T. Gill   +9 more
doaj   +2 more sources

A Protocol for Production of Mutant Mice Using Chemically Synthesized crRNA/tracrRNA with Cas9 Nickase and FokI-dCas9

Bio-Protocol, 2017
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is the most widely used genome editing tool.
Satoshi Hara, Miho Terao, Shuji Takada   +2 more
doaj   +2 more sources

Spatially blocked split CRISPR-Cas12a system for ultra-sensitive and versatile small molecule activation and detection

Nature Communications
Detecting small molecules is pivotal across fields like clinical diagnostics, environmental monitoring, and food safety. The CRISPR-Cas12a system, known for its simplicity and sensitivity, offers a promising basis for small molecule detection.
Hao Hu, Songcheng Guo, Yiyuan Li, Kejun Dong, Yan Lu, Keyi Ye, Longjie Li, Xiaoyu Zhou, Liming Cheng, Xianjin Xiao   +9 more
doaj   +2 more sources

Direct and accurate miRNA detection based on CRISPR/Cas13a-triggered exonuclease-iii-assisted colorimetric assay

Journal of Analytical Science and Technology
The abnormally expressed microRNAs (miRNAs) serve as crucial indicators for disease diagnosis and are strongly associated with the progression of various diseases.
Yunxiao Li, Qiuxia Wang, Yali Wang
doaj   +2 more sources

Cas9 senses CRISPR RNA abundance to regulate CRISPR spacer acquisition. [PDF]

Nature
Prokaryotes create adaptive immune memories by acquiring foreign DNA snippets, known as spacers, into the CRISPR array1. In type II CRISPR–Cas systems, the RNA-guided effector Cas9 also assists the acquisition machinery by selecting spacers from the ...
Zhou X, Diao R, Li X, Ziegler CA, Gramelspacher MJ, Freddolino L, Hou Z, Zhang Y.   +7 more
europepmc   +2 more sources

Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics

iScience, 2021
Summary: Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes bound to CRISPR RNA (crRNA) recognize specific nucleic acid targets, initiating ...
Eric A. Nalefski, Nidhi Patel, Philip J.Y. Leung, Zeba Islam, Remy M. Kooistra, Ishira Parikh, Estelle Marion, Gavin J. Knott, Jennifer A. Doudna, Anne-Laure M. Le Ny, Damian Madan   +10 more
doaj   +1 more source

Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes

Cell Reports, 2020
Summary: In CRISPR-Cas9-assisted knockin (KI) in zygotes, a remaining challenge is routinely achieving high-efficiency KI of large (kilobase-sized) DNA elements.
Takaya Abe, Ken-ichi Inoue, Yasuhide Furuta, Hiroshi Kiyonari   +3 more
doaj   +1 more source